The effect of sustained and local administration of epidermal growth factor on improving bilateral testicular tissue after torsion

Uguralp, S
Karabulut, AB
Mizrak, B
Kaymaz, F
Kiziltay, A
Hasırcı, Nesrin
Epidermal growth factor (EGF) modulates Leydig cell proliferation, steroidogenesis, spermiogenesis, and Sertoli cell activity. It plays an important role in repairing ischemia-reperfusion injury in different tissues. The aim of this study was to evaluate the effects of sustained and local administration of EGF on improving bilateral testicular tissue after torsion. A total of 57 Wistar albino rats were used. For the EGF transport system, 1x2 cm gelatin films containing 2 mug EGF were used. Torsion was created by rotating the right testis 720degrees in a clockwise direction for 4 h in all groups except the control group. Then, in the torsion group, bilateral orchiectomy was performed. After returning the torsioned ipsilateral testes to their normal state, the bilateral testes were wrapped by 1x2 cm unloaded gelatin films in the gelatin (G7 and G21) groups and, by 2 mug EGF loaded gelatin films in the EGF 7 and EGF 21 groups. The testes were removed on the seventh and 21st days, respectively, for biochemical and histological examination. Histologically, Johnsen's spermatogenesis criteria and mean seminiferous tubule diameter (MSTD) measurements were used. The EGF7 group did not show significant loss of Sertoli cells, while in the G7 group the number of these cells decreased. The ipsilateral ischemic testis of the EGF21 group showed Leydig cell hyperplasia, and the contralateral non-ischemic testes in this group were similar to the control group. In the G21 group, the bilateral testes showed Sertoli cell only syndrome in some sections, and most of the cells were undergoing apoptosis. The mean spermatogenesis scores and MSTD in the EGF7 and EGF21 groups were higher than in the G7 and G21 groups (P<0.05). Malondialdehyde levels were significantly lower in the EGF groups than in the G groups (P<0.05). Glutathione peroxidase (GSH-Px) levels in the G21 group were significantly higher than in the EGF21 group. Our study shows that local and sustained EGF release after testicular torsion improves bilateral testicular injury. EGF administration may be a new treatment choice for bilaterally injured testis after detorsion without removing the twisted testis.


The effects of insertional mutations in comQ, comP, srfA, spo0H, spo0A and abrB genes on bacilysin biosynthesis in Bacillus subtilis
Karatas, AY; Cetin, S; Özcengiz, Gülay (Elsevier BV, 2003-04-15)
In Bacillus subtilis, two extracellular signaling peptides, ComX pheromone and CSF (competence and sporulation factor), stimulate the development of genetic competence and surfaction biosynthesis in response to high cell density (quorum sensing) by regulating the activity of transcription factor ComA. We recently showed that biosynthesis of dipeptide antibiotic bacilysin is linked to ComA and PhrC(CSF) in a Spo0K(Opp)-dependent manner by constructing phrC-, comA- and oppA-disrupted mutants of B. subtilis. I...
The Effects of Local and Systemic Growth Hormone Treatment on Germ Cell Population and Fertility in an Experimental Unilateral Testicular Torsion and Orchiectomy Model
ATEŞ, UFUK; Gollu, Gulnur; Bingol-Kologlu, Meltem; BİLLUR, DENİZ; Kucuk, Gonul; Oruklu, Nihan; Bagriacik, Umit; Hasırcı, Nesrin; DİNDAR, HÜSEYİN (2015-12-01)
Purpose: We evaluated the effects of local and systemic growth hormone on the germ cell population of the contralateral testes of pubertal rats subjected to unilateral testicular torsion and orchiectomy 24 hours later.
The effect of mechanical forces on adipogenic differentiation
Sharafi, Parisa; Tezcaner, Ayşen; Department of Engineering Sciences (2008)
Numerous intra and extra cellular factors take role in differentiation of cell towards a given lineage. These factors have crucial role in cell-cell and cell-environment interactions. In this study, the aim is to investigate the effect of mechanical forces on the adipogenic differentiation of preadipocytes and mesenchymal stem cells in an in vitro model. Human preadipocytes and mesenchymal stem cells were embedded in 2 % agarose discs. According to the stress-relaxation test results it was observed that ini...
Evaluation of methylation profiles of an epidermal growth factor receptor gene in a head and neck squamous cell carcinoma patient group
Mutlu, M.; Mutlu, Pelin; Azarkan, S.; Baylr, Ö.; Öcal, B.; Saylam, G.; KORKMAZ, MEHMET HAKAN (2021-03-23)
Upregulation of the epidermal growth factor receptor (EGFR) gene has shown an important impact on the development of head and neck cancers due to its important regulation role on multiple cell signaling pathways. The aim of this study was to investigate the methylation pattern of the promoter region of the EGFR gene between head and neck squamous cell carcinoma (HNSCC) patients and a control group. Forty-seven unrelated HNSCC patients, clinically diagnosed at the Department of Otorhinolaryngology, Dlşkapl Y...
The effects of palbociclib on 2d and 3d cell cultures and doxorubicin resistance
Yalçın, Gizem Damla; Gündüz, Ufuk; Department of Molecular Biology and Genetics (2020)
Cell culture facilitates studying the mechanism of diseases and finding new treatment strategies. Traditionally used two­dimensional cell culture model is not quite sufficient in some studies due to the factors such as lack of cell to cell and cell­extracellular matrix interactions and changing cell morphology. Because of the limitations in 2D cell culture, nowadays three­dimensional cell cultures gain importance. The present study aims to identify the effect of Palbociclib on different cells when they cult...
Citation Formats
S. Uguralp, A. Karabulut, B. Mizrak, F. Kaymaz, A. Kiziltay, and N. Hasırcı, “The effect of sustained and local administration of epidermal growth factor on improving bilateral testicular tissue after torsion,” UROLOGICAL RESEARCH, pp. 323–331, 2004, Accessed: 00, 2020. [Online]. Available: