Evaluation of methylation profiles of an epidermal growth factor receptor gene in a head and neck squamous cell carcinoma patient group

2021-03-23
Mutlu, M.
Mutlu, Pelin
Azarkan, S.
Baylr, Ö.
Öcal, B.
Saylam, G.
KORKMAZ, MEHMET HAKAN
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
395
views
44
downloads
Upregulation of the epidermal growth factor receptor (EGFR) gene has shown an important impact on the development of head and neck cancers due to its important regulation role on multiple cell signaling pathways. The aim of this study was to investigate the methylation pattern of the promoter region of the EGFR gene between head and neck squamous cell carcinoma (HNSCC) patients and a control group. Forty-seven unrelated HNSCC patients, clinically diagnosed at the Department of Otorhinolaryngology, Dlşkapl Ylldlrlm Beyazlt Training and Research Hospital, Ankara, Turkey, and 48 unrelated healthy volunteers from different geographic regions of Turkey, were included in this study. Methylation status of the promoter region of the EGFR gene was detected by methylation-specific-polymerase chain reaction (MS-PCR). The correlation between EGFR gene promoter methylation profiles and clinical characteristics were examined using the χ2 test. Methylation was observed in 79.0% of HNSCC patients, whereas this ratio was 90.0% in healthy individuals. The results show that promoter region methylation of the EGFR gene was not associated with HNSCC development in the studied Turkish patient group. In addition, the methylation status of the EGFR gene promoter was not found to be related to age, gender or tumor stage.
Epidermal growth factor receptor (EFGR) gene, Epigenetics, Head and neck squamous cell carcinoma (HNSCC), Methylation, Methylation-specific-polymerase chain reaction (MS-PCR)
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85103189641&origin=inward
https://hdl.handle.net/11511/90849
Balkan Journal of Medical Genetics
Test and Measurement Center In advanced Technologies (MERKEZ LABORATUVARI), Article

Suggestions

Investigation of the role of programmed cell death 10 (PDCD10) protein in multidrug resistance
Urfalı Mamatoğlu, Çağrı; Gündüz, Ufuk; Department of Biology (2018)
Drug resistance, a major obstacle in chemotherapy, is the sum of several cellular alterations including resistance to induction of apoptosis. Apoptosis is a well-regulated cell death mechanism which is controlled by several signaling pathways and a vast number of proteins. Alterations in the proteins involved in the apoptotic regulation have been associated with drug resistance in cancer. Programmed Cell Death 10 (PDCD10) protein is a novel apoptotic regulator that is recently linked to the modulation of ce...
Evaluation of neointimal hyperplasia on tranilast-coated synthetic vascular grafts: An experimental study
Karakayali, Feza; Haberal, Nihan; Tufan, Hale; Hasırcı, Nesrin; BaSaran, Ozgur; Sevmis, Sinasi; Akdur, Aydin; Kızıltay, Aysel; Haberal, Mehmet (2007-01-01)
Tranilast is an antiallergic drug that interferes with proliferation and migration of vascular smooth muscle cell induced by platelet-derived growth factor (PDGF) and transforming growth factor-beta 1 (TGF-beta 1). We investigated the local effect of tranilast on neointimal hyperplasia using tranilastcoated prosthetic grafts. The inner sides of the thin-walled polytetrafluoroethylene (PTFE) grafts were coated with chitosan and tranilast containing chitosan solution. Wistar albino rats (32) were used in the ...
DETERMINATION OF THE RELATIONSHIP BETWEEN DOXORUBICIN RESISTANCE AND WNT SIGNALING PATHWAY IN HELA AND K562 CELL LINES
Mutlu, Pelin; Pourianazar, Negar Taghavi; Yucel, Meral; Gündüz, Ufuk (2018-01-01)
Activation of the Wnt signaling in some types of cancer and its relation with chemotherapy resistance is a very interesting issue that has been emphasized in recent years. Although, it is known that increase in the activity of beta-catenin is important in blast transformation and drug resistance, the underlying mechanisms are still unclear. In this study, changes in the expression levels of 186 genes that are thought to be important in drug resistance and Wnt signaling pathways were determined by using qPCR...
Investigation of the molecular pathways involved in intestinal epithelial differentiation
Sade Memişoğlu, Aslı; Banerjee, Sreeparna; Department of Biology (2014)
The molecular mechanisms of balanced and continuous generation of intestinal epithelial cells, is poorly understood and disruption of this balance may result in neoplastic transformation and malignant growth. Differentiation is regulated by numerous signals, which in turn regulate signaling pathways directing activation or inactivation of certain transcription factors. Perturbations like changes in Ca2+ levels, glucose or amino acid starvation result in an ER stress response, which is also implicated in the...
Investigation of docetaxel and doxorubicin resistance in mcf-7 breast carcinoma cell line
Darcansoy İşeri, Özlem; Gündüz, Ufuk; Department of Biotechnology (2009)
Multidrug resistance phenotype of tumor cells describes resistance to wide range of structurally unrelated anticancer agents and is a serious limitation to effective chemotherapy. It is a multifactor yet not fully elucidated phenomenon by the involvement of diverse cellular pathways. Aim of this study was to investigate the resistance mechanisms developed against docetaxel and doxorubicin that are widely used in the treatment of breast cancer in model cell line MCF-7. Resistant sublines were developed by ap...
Citation Formats
M. Mutlu et al., “Evaluation of methylation profiles of an epidermal growth factor receptor gene in a head and neck squamous cell carcinoma patient group,” Balkan Journal of Medical Genetics, pp. 65–72, 2021, Accessed: 00, 2021. [Online]. Available: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85103189641&origin=inward.
METU IIS - Integrated Information Service open@metu.edu.tr