Peptidomic characterization and bioactivity of Protoiurus kraepelini (Scorpiones: Iuridae) venom

Date

2018-01-01

Author

Doğan, Tuğba
BİBER, Aysenur
GEREKCİ, Selin
Hüsnügil, Hepşen Hazal
Özen, Can

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Protoiurus kraepelini is a scorpion species found in parts of Turkey and Greece. In this study, the peptide profile of its venom was determined for the first time. The electrophoretic profile of the crude venom showed a protein distribution from 2 to 130 kDa. MALDI-TOF MS analysis of the venom peptide fraction yielded 27 peptides between 1059 and 4623 Da in mass. Several ion channelblocking and antimicrobial peptides were identified by peptide mass fingerprinting analysis. Cytotoxic and antimicrobial effects of the venom were also demonstrated on Jurkat cells and Escherichia coli, respectively. As the first peptidomic characterization study on P. kraepelini venom, this report lays the foundation for detailed future studies that may lead to the discovery of novel bioactive peptides.

Subject Keywords

Scorpion venom, Peptide, Peptidomics, Antimicrobial effect, Cytotoxicity, Mass spectrometry

URI

https://hdl.handle.net/11511/30419

Journal

TURKISH JOURNAL OF BIOLOGY

DOI

https://doi.org/10.3906/biy-1804-35

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

T. Doğan, A. BİBER, S. GEREKCİ, H. H. Hüsnügil, and C. Özen, “Peptidomic characterization and bioactivity of Protoiurus kraepelini (Scorpiones: Iuridae) venom,” TURKISH JOURNAL OF BIOLOGY, pp. 490–498, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30419.