Genetic differentiation of Liquidambar Orientalis Mill. varieties with respect to matK region of chloroplast genome

Özdilek, Aslı
Liquidambar L. genus is represented with mainly 4 species in the world and one of these species, Turkish sweet gum (Liquidambar orientalis Mill.) which is a relictendemic species is naturally found in only southwestern Turkey, mainly in Muğla Province. The limited distribution of species with two disputed varieties (var. integriloba Fiori and var. orientalis) and increased anthropogenic threats to its genetic resources signify the importance of studying genetic diversity in the species to have better conservation and management programs. For this purpose, 18 different populations were sampled throughout the species range and matK region of chloroplast DNA (cpDNA) was sequenced to assess the genetic structure of the species. Turkish Liquidambar orientalis populations were evaluated at two categories: variety level and geographic level. Also, two sectors of matK region were examined to assess which part of the region was more variable. All molecular analysis was conducted in this study by using MEGA version 3.1 and Arlequin 2.000 softwares. v Moleculer diversity analysis indicated that the population located in Fethiye- Günlükbası district has the highest number of polymorphic sites. This population is also genetically the most distant from the others (average genetic distance 0.0038). Among the studied varieties, the average genetic distance within var. integriloba (0.0016) which also includes population Fethiye-Günlükbası was the greatest. Among the geographic regions, Muğla-1 including Fethiye-Köyceğiz-Aydın district as well as population Fethiye-Günlükbası showed the highest average genetic distances within the region with a value of 0.0015. According to the molecular variance results, among varieties and among geographic regions, there was no significant differentiation, but great amount of total variation was found (~86%) within Turkish sweet gum populations. With respect to the Fst values among varieties, the highest genetic differentiation was observed between var. orientalis and unknown group (0.040). Furthermore, based on the results of phylogenetic analysis, Turkish populations of L. orientalis have genetically closer to USA relative (L. styraciflua L.) than Chinese relatives (L. acalycina H.T Chang and L. formosana Hance). In conclusion, 10 Turkish sweet gum populations were found to be important for conservation issues. Furthermore, eight of these located in Muğla province and sixth of them belong to var. integriloba. Especially Fethiye-Günlükbası, MarmarisÇetibeli and Muğla-Kıyra populations should be included in either insitu or exsitu or in both conservation programs in the future.


Genetic characterization pinus nigra subspecies pallasiana varieties, natural populations (seed stands), seed orchards and plantations
Çengel (Nazlıer), Burcu; Kaya, Zeki; Department of Biology (2005)
Pinus nigra subsp. pallasiana is one of the most widespread and economically important forest tree species in Turkey. Primary objective of the present study was to to reveal the effects of forestry practices by determining genetic diversity of natural and managed seed sources by means of RAPD markers. Secondly, two varieties were also investigated to reveal their pattern of genetic variation. Seed stands, seed orchards and plantations were screened against 11 RAPD primers and generated 152 polymorphic DNA l...
Polymorphism of Prolactin (PRL), Diacylglycerol Acyltransferase (DGAT-1) and Bovine Solute Carrier Family 35 Member 3 (SLC35A3) genes in native cattle breeds and its implication for Turkish cattle breeding
Kepenek, Eda Şeyma; Togan, İnci Zehra; Department of Biology (2007)
In the present study samples from four native Turkish Cattle Breeds; South Anatolian Red (n= 48), East Anatolian Red (n= 34), Anatolian Black (n= 42) and Turkish Grey (n=46) and elite bulls of Holstein (n=21) were genotyped with respect to two milk production enhancer genes, Prolactin (PRL) and Diacylglycerol acyltransferase (DGAT1), and one disease (Complex Vertebral Malformation) causing gene (SLC35A3). A allele frequency for PRL gene, believed to be positively associated with the milk yield in cattle, ra...
The phylogenetic analysis of pinus nigra arnold subspecies pallasiana varieties with respect to non-coding trn regions of chloroplast genome
Güvendiren Gülsoy, Aysun Demet; Kaya, Zeki; Department of Biology (2009)
More than half of the Pinaceae is including in genus Pinus covers the large parts of vegetation of northern hemisphere. The Anatolian Black Pine is one of the subspecies of European Black Pine, growing naturally as a widespread mid elevation species of Taurus, western Anatolian and northern Anatolian Mountains of Turkey. Although it is disputed that there are 5 varieties of Anatolian black pine but three of these are well recognized. These are Pinus nigra subsp. pallasiana var. pallasiana, Pinus nigra Arnol...
Genetic impacts of Anacapa deer mice reintroductions following rat eradication
Ozer, Fusun; Gellerman, Holly; Ashley, Mary V. (Wiley, 2011-09-01)
The Anacapa deer mouse is an endemic subspecies that inhabits Anacapa Island, part of Channel Islands National Park, California. We used mitochondrial DNA cytochrome c oxidase subunit II gene (COII) and 10 microsatellite loci to evaluate the levels of genetic differentiation and variation in similar to 1400 Anacapa deer mice sampled before and for 4 years after a black rat (Rattus rattus) eradication campaign that included trapping, captive holding and reintroduction of deer mice. Both mitochondrial and mic...
Genetic diversity of scald (rhynchosporium secalis) disease resistant and sensitive Turkish barley seed sources as determined with simple sequence repeats
Dizkırıcı, Ayten; Kaya, Zeki; Department of Biology (2006)
Scald disease (Rhynchosporium secalis) is one of the major plant diseases causing considerable yield loss in barley (Hordeum vulgare) plantations in Turkey. To develop, scald resistant barley varieties, C.R.I.F.C. of Turkey has a large accumulated collection of barley seed sources in hand, but these samples are difficult to be followed and used in the breeding programs due to lack of genetic studies on them. Thus, the objective of this study was to characterize and fingerprint of eighty barley seed sources,...
Citation Formats
A. Özdilek, “Genetic differentiation of Liquidambar Orientalis Mill. varieties with respect to matK region of chloroplast genome,” M.S. - Master of Science, Middle East Technical University, 2007.