Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
vanA-targeted oligonucleotide DNA probe designed to monitor vancomycin- and teicoplanin-resistant bacteria in surface waters
Date
2016-10-01
Author
Nakipoğlu, Mustafa
İçgen, Bülent
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
178
views
0
downloads
Cite This
The glycopeptide antibiotics teicoplanin and vancomycin are common to treat severe Gram-positive bacterial infections. The gene vanA confers high-level resistance to these antibiotics, and these phenomena have been shown to be transferable. Release of vancomycin-and teicoplanin-resistant bacteria to surface waters is, therefore, of particular concern since they might proliferate and spread in different environments. Monitoring of the fate of vanA gene in these waters provides information on the exposure and potential threats of those bacteria for the environment and public health. Therefore, this study aimed at preparing a 25-mer-oligonucleotide DNA probe based on the 909 bp BamHI-ClaI fragment from Enterococcus faecium plasmids pVEF1 and pVEF2 through the use of Vector NTI Express Software. The newly designed vanA probe displayed highly specific hybridization with vanA-positive Enterococcus faecalis tested at 46 degrees C, 55 % formamide, and 0.020 M NaCl stringency conditions. In situ fluorescein hybridizations under the same stringency conditions were also used to monitor river water samples by using fluorescein microscopy. The results showed that the vanA-targeted oligonucleotide DNA probe prepared was not only highly specific but also quantitative tool for monitoring vancomycin-and teicoplanin-resistant bacteria in surface waters.
Subject Keywords
Glycopeptide resistance
,
Vancomycin
,
Teicoplanin
,
vanA DNA probe
,
Surface waters
URI
https://hdl.handle.net/11511/30585
Journal
ENVIRONMENTAL MONITORING AND ASSESSMENT
DOI
https://doi.org/10.1007/s10661-016-5578-7
Collections
Graduate School of Natural and Applied Sciences, Article
Suggestions
OpenMETU
Core
vanA Gene Harboring Enterococcal and Non-enterococcal Isolates Expressing High Level Vancomycin and Teicoplanin Resistance Reservoired in Surface Waters
Nakipoğlu, Mustafa; İçgen, Bülent (2017-05-01)
Untreated wastewaters and treated effluents even after final disinfection contain antibiotic resistant bacteria and resistance genes before they are released into surface waters. A correlation between resistant bacteria and antibiotics in surface waters has been found, as have antibiotic resistance genes. Of particular interest are vancomycin-resistant enterococci harboring vanA gene that confers high level of resistance to glycopeptide antibiotics including teicoplanin. Therefore, in this study, river wate...
VanA-Type MRSA (VRSA) Emerged in Surface Waters
İçgen, Bülent (Springer Science and Business Media LLC, 2016-09-01)
Due to the widespread occurrence of mecA-encoded methicillin resistance in Staphylococcus aureus (MRSA), treatment of staphylococcal infections is shifted to glycopeptide antibiotics like vancomycin and teicoplanin. The selective pressure of glycopeptides has eventually led to the emergence of staphylococci with increased resistance. Of great concern is vanA-encoded high level vancomycin and teicoplanin resistance in MRSA (VRSA). Therefore, this study aimed at investigating the occurrence of VRSA in surface...
vanA harboring enterococcal and non-enterococcal surface water isolates monitored by an oligonucleotide DNA probe /
Nakipoğlu, Mustafa; İçgen, Bülent; Çetin, Sema; Department of Biotechnology (2016)
Untreated wastewaters and treated effluents even after final disinfection inhabitate antibiotic resistant bacteria and resistance genes before they are released into surface waters. A correlation between resistant bacteria with antibiotic resistance genes in surface waters has been found. Of particular interest are vancomycin-resistant enterococci (VRE) harboring vanA gene that confers their high-level resistance to glycopeptide antibiotics including teicoplanin. Therefore, in this study, river water sample...
MEMS based electrochemical DNA sensor to detect methicillin resistant Staphylococcus aureus and vancomycin resistant Enterococcus species
Ceylan Koydemir, Hatice; Özgen, Canan; Külah, Haluk; Department of Chemical Engineering (2013)
Methicillin Resistant Staphylococcus aureus (MRSA) is one of the most important threats of nosocomial infections in many regions of the world and Vancomycin Resistant Enterococcus (VRE) is an emerging pathogen that develops full resistance against third-generation glycopeptide antibiotics. Conventional methods for identification of MRSA and VRE generally depend on culturing, which requires incubation of biological samples at least 24-72 hours to get accurate results. These methods are time consuming and nec...
Development and analysis of controlled release polymeric rods containing vancomycin
Tağıt, Oya; Hasırcı, Vasıf Nejat; Department of Biotechnology (2005)
Antibiotic use is a vital method for the treatment of most diseases involving bacterial infections. Unfortunately, in certain cases these agents are not effective in treatments against diseases for either some limitation in antibiotic usage because of the side effects or some distribution problems caused by physiological or pathological barriers in the body. Such problems are thought to be minimized by development of controlled release systems which involve implantation of antibiotic loaded polymeric system...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. Nakipoğlu and B. İçgen, “vanA-targeted oligonucleotide DNA probe designed to monitor vancomycin- and teicoplanin-resistant bacteria in surface waters,”
ENVIRONMENTAL MONITORING AND ASSESSMENT
, pp. 0–0, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30585.