vanA-targeted oligonucleotide DNA probe designed to monitor vancomycin- and teicoplanin-resistant bacteria in surface waters

Date

2016-10-01

Author

Nakipoğlu, Mustafa
İçgen, Bülent

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The glycopeptide antibiotics teicoplanin and vancomycin are common to treat severe Gram-positive bacterial infections. The gene vanA confers high-level resistance to these antibiotics, and these phenomena have been shown to be transferable. Release of vancomycin-and teicoplanin-resistant bacteria to surface waters is, therefore, of particular concern since they might proliferate and spread in different environments. Monitoring of the fate of vanA gene in these waters provides information on the exposure and potential threats of those bacteria for the environment and public health. Therefore, this study aimed at preparing a 25-mer-oligonucleotide DNA probe based on the 909 bp BamHI-ClaI fragment from Enterococcus faecium plasmids pVEF1 and pVEF2 through the use of Vector NTI Express Software. The newly designed vanA probe displayed highly specific hybridization with vanA-positive Enterococcus faecalis tested at 46 degrees C, 55 % formamide, and 0.020 M NaCl stringency conditions. In situ fluorescein hybridizations under the same stringency conditions were also used to monitor river water samples by using fluorescein microscopy. The results showed that the vanA-targeted oligonucleotide DNA probe prepared was not only highly specific but also quantitative tool for monitoring vancomycin-and teicoplanin-resistant bacteria in surface waters.

Subject Keywords

Glycopeptide resistance, Vancomycin, Teicoplanin, vanA DNA probe, Surface waters

URI

https://hdl.handle.net/11511/30585

Journal

ENVIRONMENTAL MONITORING AND ASSESSMENT

DOI

https://doi.org/10.1007/s10661-016-5578-7

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

M. Nakipoğlu and B. İçgen, “vanA-targeted oligonucleotide DNA probe designed to monitor vancomycin- and teicoplanin-resistant bacteria in surface waters,” ENVIRONMENTAL MONITORING AND ASSESSMENT, pp. 0–0, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30585.