Towards a super H-2 producer: Improvements in photofermentative biohydrogen production by genetic manipulations

2010-07-01
KARS, GÖKHAN
Gündüz, Ufuk
Photofermentative hydrogen production by purple non-sulfur bacteria is a potential candidate among biological hydrogen production methods. Hydrogen is produced under anaerobic conditions in light using different organic substrates as carbon source. The hydrogen evolution occurs mainly through the catalytic activity of the nitrogenases under non-repressive concentrations of ammonia. However, total hydrogen production is constrained due to several reasons in purple non-sulfur (PNS) bacteria, such as consumption of hydrogen by uptake hydrogenase, inefficient hydrogen production capacity of nitrogenase, limited electron flow to the nitrogenase, sensitivity of nitrogenase towards ammonia, etc. Hence, PNS bacteria need to be manipulated genetically to overcome these limitations and to make the process practically feasible. This review focuses on various approaches for the genetic improvement of biohydrogen production by PNS bacteria. (C) 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY

Suggestions

Evaluation of hydrogen production by Rhodobacter sphaeroides OU001 and its hupSL deficient mutant using acetate and malate as carbon sources
KARS, GÖKHAN; Gündüz, Ufuk; Yucel, Meral; Rakhely, Gabor; Kovacs, Kornel L.; Eroglu, Inci (2009-03-01)
Rhodobacter sphaeroides O.U.001 is one of the candidates for photobiological hydrogen production among purple non-sulfur bacteria. Hydrogen is produced by Mo-nitrogenase from organic acids such as malate or lactate. A hupSL in frame deletion mutant strain was constructed without using any antibiotic resistance gene. The hydrogen production potential of the R. sphaeroides O.U.001 and its newly constructed hupSL deleted mutant strain in acetate media was evaluated and compared with malate containing media. Th...
Potential use of thermophilic dark fermentation effluents in photofermentative hydrogen production by Rhodobacter capsulatus
Ozgur, Ebru; Afsar, Nilufer; de Vrije, Truus; Yucel, Meral; Gündüz, Ufuk; Claassen, Pieternel A. M.; Eroglu, Inci (2010-01-01)
Biological hydrogen production by a sequential operation of dark and photofermentation is a promising route to produce hydrogen. The possibility of using renewable resources, like biomass and agro-industrial wastes, provides a dual effect of sustainability in biohydrogen production and simultaneous waste removal. In this study, photofermentative hydrogen production on effluents of thermophilic dark fermentations on glucose, potato steam peels (PSP) hydrolysate and molasses was investigated in indoor, batch ...
Hydrogen production by hup(-) mutant and wild-type strains of Rhodobacter capsulatus from dark fermentation effluent of sugar beet thick juice in batch and continuous photobioreactors
Uyar, Basar; Gurgan, Muazzez; Ozgur, Ebru; Gündüz, Ufuk; Yücel, Ayşe Meral; Eroglu, Inci (2015-10-01)
Photofermentative production of hydrogen is a promising and sustainable process; however, it should be coupled to dark fermentation to become cost effective. In order to integrate dark fermentation and photofermentation, the suitability of dark fermenter effluents for the photofermentative hydrogen production must be demonstrated. In this study, thermophilic dark fermenter effluent (DFE) of sugar beet thick juice was used as a substrate in photofermentation process to compare wild-type and uptake hydrogenas...
Biological hydrogen production by using co-cultures of PNS bacteria
Baysal, Görkem; Yücel, Ayşe Meral; Özgür, Ebru; Department of Biotechnology (2012)
Biological hydrogen production is a renewable, carbon-neutral and clean route for hydrogen production. Purple non-sulfur (PNS) bacteria have the ability to produce biohydrogen via photofermentation process. The type of the bacterial strain used in photofermentation is known to have an important effect on hydrogen yield. In this study, the effect of different co-cultures of PNS bacteria on photofermentation process was investigated in search of improving the hydrogen yield. For this purpose, growth, hydrogen...
Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides
Koku, Harun (Elsevier BV, 2002-11)
Photosynthetic bacteria are favorable candidates for biological hydrogen production due to their high conversion efficiency and versatility in the substrates they can utilize. For large-scale hydrogen production, an integrated view of the overall metabolism is necessary in order to interpret results properly and facilitate experimental design. In this study, a summary of the hydrogen production metabolism of the photosynthetic purple non-sulfur (PNS) bacteria will be presented. Practically all hydrogen prod...
Citation Formats
G. KARS and U. Gündüz, “Towards a super H-2 producer: Improvements in photofermentative biohydrogen production by genetic manipulations,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 6646–6656, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31045.