Hydrogen production by hup(-) mutant and wild-type strains of Rhodobacter capsulatus from dark fermentation effluent of sugar beet thick juice in batch and continuous photobioreactors

Date

2015-10-01

Author

Uyar, Basar
Gurgan, Muazzez
Ozgur, Ebru
Gündüz, Ufuk
Yücel, Ayşe Meral
Eroglu, Inci

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Photofermentative production of hydrogen is a promising and sustainable process; however, it should be coupled to dark fermentation to become cost effective. In order to integrate dark fermentation and photofermentation, the suitability of dark fermenter effluents for the photofermentative hydrogen production must be demonstrated. In this study, thermophilic dark fermenter effluent (DFE) of sugar beet thick juice was used as a substrate in photofermentation process to compare wild-type and uptake hydrogenase-deficient (hup (-)) mutant strains of Rhodobacter capsulatus by means of hydrogen production and biomass growth. The tests were conducted in small-scale (50 mL) batch and large-scale (4 L) continuous photobioreactors in indoor conditions under continuous illumination. In small scale batch conditions, maximum cell concentrations were 0.92 gdcw/L (c) and 1.50 gdcw/L (c), hydrogen yields were 34 % and 31 %, hydrogen productivities were 0.49 mmol/(L (c)center dot h) and 0.26 mmol/(L-c center dot h), for hup (-) and wild-type cells, respectively. In large-scale continuous conditions, maximum cell concentrations were 1.44 gdcw/L (c) and 1.87 gdcw/L (c), hydrogen yields were 48 and 46 %, and hydrogen productivities were 1.01 mmol/(L (c)center dot h) and 1.05 mmol/(L (c)center dot h), for hup (-) and wild-type cells, respectively. Our results showed that Rhodobacter capsulatus hup (-) cells reached to a lower maximum cell concentration but their hydrogen yield and productivity were in the same range or superior compared to the wild-type cells in both batch and continuous operating modes. The maximum biomass concentration, yield and productivity of hydrogen were higher in continuous mode compared to the batch mode with both bacterial strains.

Subject Keywords

Sugar beet thick juice, Rhodobacter capsulatus, Photofermentation, Biohydrogen

URI

https://hdl.handle.net/11511/31629

Journal

BIOPROCESS AND BIOSYSTEMS ENGINEERING

DOI

https://doi.org/10.1007/s00449-015-1435-2

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

B. Uyar, M. Gurgan, E. Ozgur, U. Gündüz, A. M. Yücel, and I. Eroglu, “Hydrogen production by hup(-) mutant and wild-type strains of Rhodobacter capsulatus from dark fermentation effluent of sugar beet thick juice in batch and continuous photobioreactors,” BIOPROCESS AND BIOSYSTEMS ENGINEERING, pp. 1935–1942, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31629.