Potential use of thermophilic dark fermentation effluents in photofermentative hydrogen production by Rhodobacter capsulatus

Date

2010-01-01

Author

Ozgur, Ebru
Afsar, Nilufer
de Vrije, Truus
Yucel, Meral
Gündüz, Ufuk
Claassen, Pieternel A. M.
Eroglu, Inci

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Biological hydrogen production by a sequential operation of dark and photofermentation is a promising route to produce hydrogen. The possibility of using renewable resources, like biomass and agro-industrial wastes, provides a dual effect of sustainability in biohydrogen production and simultaneous waste removal. In this study, photofermentative hydrogen production on effluents of thermophilic dark fermentations on glucose, potato steam peels (PSP) hydrolysate and molasses was investigated in indoor, batch operated bioreactors. An extreme thermophile Caldicellulosiruptor saccharolyticus was used in the dark fermentation step, and Rhodobacter capsulatus (DSM1710) was used in the photofermentation step. Addition of buffer, Fe and Mo to dark fermentor effluents (DFEs) improved the overall efficiency of hydrogen production. The initial acetate concentration in the DFE needed to be adjusted to 30-40 mM by dilution to increase the yield of hydrogen in batch light-supported fermentations. The thermophilic DFEs are suitable for photofermentative hydrogen production, provided that they are supplemented with buffer and nutrients. The overall hydrogen yield of the two-step fermentations was higher than the yield of single step dark fermentations.

Subject Keywords

Rhodobacter capsulatus, Biohydrogen, Molasses, Potato steam peels, Dark fermentation, Photofermentation

URI

https://hdl.handle.net/11511/30300

Journal

JOURNAL OF CLEANER PRODUCTION

DOI

https://doi.org/10.1016/j.jclepro.2010.02.020

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

E. Ozgur et al., “Potential use of thermophilic dark fermentation effluents in photofermentative hydrogen production by Rhodobacter capsulatus,” JOURNAL OF CLEANER PRODUCTION, pp. 0–0, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30300.