Poly-beta-hydroxybutyrate production by lactic acid bacteria

Date

1998-02-15

Author

Aslim, B
Caliskan, F
Beyatli, Y
Gündüz, Ufuk

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

54
views

0
downloads

Poly-beta-hydroxybutyrate was determined in lactic acid bacteria belonging to the genera Lactobacillus, Lactococcus, Pediococcus and Streptococcus. Lactobacilli were grown in MRS broth, the others were grown in Elliker broth medium. Cell biomass was obtained by centrifugation. The cell walls were lysed with sodium hypochlorite. Poly-beta-hydroxybutyrate was extracted using chloroform in a Soxhlet system. Then it was converted to crotonic acid using sulfuric acid and the amount of crotonic acid was measured spectrophotometrically. The yield of poly-beta-hydroxybutyrate (% of cell dry weight) of Lactobacillus species was 6.6-35.8%. The values for Lactococcus, Pediococcus and Streptococcus species were 9.0-20.9, 1.1-8.0 and 6.8-17.2, respectively. It was observed that one of the Lactobacillus species did not produce poly-beta-hydroxybutyrate. Generally, Lactobacillus species produced more poly-beta-hydroxybutyrate than the other tested bacteria and no significant correlation was observed between poly-beta-hydroxybutyrate production and cell density of the cultures. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

Subject Keywords

Lactobacillus, Lactococcus, Pediococcus, Streptococcus, Poly-beta-hydroxybutyrate production

URI

https://hdl.handle.net/11511/31448

Journal

FEMS MICROBIOLOGY LETTERS

DOI

https://doi.org/10.1016/s0378-1097(97)00557-0

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Poly(hydroxybutyrate-co-hydroxyvalerate) nanocapsules as enzyme carriers for cancer therapy: an in vitro study Baran, ET; Ozer, N; Hasırcı, Vasıf Nejat (2002-05-01) In the present paper, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanocapsules were prepared by a double emulsion-solvent evaporation procedure (w/o/w) for the encapsulation of model enzymes (L-asparaginase, catalase, glucose oxidase) and bovine serum albumin. To increase the encapsulation efficiency and activity of the encapsulated enzyme, numerous modifications were made in the compositions of the phases of double emulsion. For the preparation of low molecular weight PHBV, the polymer was treated with so...
Immune modulatory effects of pediococcus pentosaceus derived membrane vesicles: mechanism of action and therapeutic applications Alpdündar Bulut, Esin; Gürsel, Mayda; Department of Biology (2018) In our previous studies, we characterized 5 different human gram positive commensal bacteria derived membrane vesicles (MVs) and compared their activity with non-pathogenic E.coli derived membrane vesicles. Results showed that commensal bacteria derived MVs had immunomodulatory properties whereas non-pathogenic E.coli derived membrane vesicles had immune stimulatory properties. In this thesis, we aimed to focus our attention to Pediococcus pentosaceus-derived MVs that displayed the highest immunomodulatory ...
Poly-beta-hydroxybutyrate accumulation and releasing by hydrogen producing bacteria, Rhodobacter sphaeroides OU001. A transmission electron microscopic study Cetin, Demet; Guenduez, Ufuk; Eroğlu, İnci; Yuecel, Meral; Tuerker, Lemi (2006-11-16) Photosynthetic bacterium Rhodobacter sphaeroides O.U.001 that is used for photobiohydrogen production can also accumulate poly-beta-hydroxybutyrate (PHB) as a by-product when cultivated anaerobically with minimal medium containing L-malic acid, sodium glutamate and some vitamins under illumination. Transmission electron microscopy studies revealed that PHB granules are made of two distinct components: a homogenous and electron lucent core is covered with a more electron dense coat. PHB granules were observe...
Heavy metal biosorption by white-rot fungi Yetiş, Ülkü; Özcengiz, Gülay; Dilek, Filiz Bengü (1998-06-26) In this study, heavy metal biosorption potentials of two white-rot fungi, Polyporous versicolor and Phanarochaete chrysosporium, which are commonly used in wastewater treatment were determined. Biosorption studies were performed for Cu(II), Cr(III), Cd(II), Ni(II) and Pb(II) at the same operational conditions and the effectiveness of both fungi at removing these heavy metals was compared. It was found that both P. versicolor and P. chrysosporium were the most effective in removing Pb(II) from aqeous solutio...
Nitrate reductase and glutamate dehydrogenase activities of resistant and sensitive cultivars of wheat and barley under boron toxicity Mahboobi, H; Yucel, M; Öktem, Hüseyin Avni (2002-01-01) In this study, changes in activity of key enzymes of nitrogen assimilation, namely nitrate reductase and glutamate dehydrogenase upon boron (B) toxicity in wheat (Triticum aestivum) and barley (Hordeum vulgare) cultivars were investigated. Ten-day-old seedlings were exposed to 10 mM boric acid for a duration of five days. Plants growing on the nutrient solutions and receiving no excess B, were maintained as controls. All experiments were conducted on leaf and root tissues of control and B-treated seedlings ...

Citation Formats

B. Aslim, F. Caliskan, Y. Beyatli, and U. Gündüz, “Poly-beta-hydroxybutyrate production by lactic acid bacteria,” FEMS MICROBIOLOGY LETTERS, pp. 293–297, 1998, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31448.