Show/Hide Menu
Hide/Show Apps
anonymousUser
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Videos
Videos
Thesis submission
Thesis submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Contact us
Contact us
Nitrate reductase and glutamate dehydrogenase activities of resistant and sensitive cultivars of wheat and barley under boron toxicity
Date
2002-01-01
Author
Mahboobi, H
Yucel, M
Öktem, Hüseyin Avni
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
7
views
0
downloads
Cite This
In this study, changes in activity of key enzymes of nitrogen assimilation, namely nitrate reductase and glutamate dehydrogenase upon boron (B) toxicity in wheat (Triticum aestivum) and barley (Hordeum vulgare) cultivars were investigated. Ten-day-old seedlings were exposed to 10 mM boric acid for a duration of five days. Plants growing on the nutrient solutions and receiving no excess B, were maintained as controls. All experiments were conducted on leaf and root tissues of control and B-treated seedlings of B-tolerant and B-sensitive cultivars. For estimation of activity of nitrate reductase, an in vivo assay was used. Compared to controls (no B treatment) activity of nitrate reductase tended to decrease (15-17%) following B toxicity in root and leaf tissues of all cultivars, however, no significant difference was observed between resistant and sensitive cultivars. Boron stress increased activity of glutamate dehydrogenase in roots and leaves of all cultivars by an average of 81% and 30%, respectively. Compared to sensitive variety, the boron tolerant wheat variety exhibited a significantly higher increase in shoot tissue GDH activity, whereas no significant difference was observed in root tissues. It was concluded that, the increase in activity of glutamate dehydrogenase could be an adaptive mechanism in these species and possibly plays a protective role under boron-stress conditions.
Subject Keywords
Nıtrogen-metabolısm
,
Hıgher-plants
,
Expressıon
,
Seedlıngs
,
Leaves
URI
https://hdl.handle.net/11511/34971
Journal
JOURNAL OF PLANT NUTRITION
DOI
https://doi.org/10.1081/pln-120006060
Collections
Department of Biology, Article
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
H. Mahboobi, M. Yucel, and H. A. Öktem, “Nitrate reductase and glutamate dehydrogenase activities of resistant and sensitive cultivars of wheat and barley under boron toxicity,”
JOURNAL OF PLANT NUTRITION
, vol. 25, no. 8, pp. 1829–1837, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/34971.