Paclitaxel Resistance in MCF-7/Pac Cell Line is Reversed Successfully by Saikosaponin A and Saikosaponin D

Gündüz, Ufuk
Cancer cells demonstrate multiple drug resistance phenotype frequently after chemotherapy. The resistance of cancer cells to various chemotherapeutic agents is defined as multiple drug resistance. The purpose of this study is to investigate the potential reversal effects of active agents, that are found high amount in plants, on resistant MCF-7 cell lines. The effects of potential MDR modulators combined with anticancer drugs were also evaluated. Flow cytometry, fluorescence microscopy and checkerboard combination assays were performed to study the reversal of drug resistance and for investigation of the antiproliferative effects of the combination of anticancer drugs with the modulators. Paclitaxel and potential MDR modulators (verapamil, saikosaponin A, D and isoquercitrin) were applied to the sublines in combination. Fluorescence accumulation levels and fractional inhibitory indices show that saikosaponin A and D are effective MDR reversal agents that may be used together with paclitaxel in drug resistant mammary carcinoma subline. In conclusion this report represents saikosaponin A and D from natural resources are valuable reagents that may improve the success of chemotherapy.


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Background: Ineffectiveness of anticancer drugs is frequently observed in cancer chemotherapy. The resistance of tumor cells to various cytotoxic drugs is defined as multidrug resistance (MDR). The purpose of this study is to investigate the potential reversal effect of some synthetic and natural chemicals on drug-resistant MCF-7 cell lines. The effects of potential MDR modulators combined with some anticancer drugs were also studied. Methods: Flow cytometry, MTT cytotoxicity assays and checkerboard combina...
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Multidrug resistance (MDR) is a major problem in success of cancer chemotherapy on tumor cell growth, limits the prolonged and effective use of chemotherapy. The use of nanomaterial based drug carriers in cancer treatment offers exciting opportunities to enhance delivery of therapeutics to the tumor site (1). This is also known as targeted drug delivery providing differential distribution of drugs to the tumor site while significantly reducing the overall toxicity. Here, we critically discuss the role of th...
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High toxicity caused by chemotherapeutic drugs and the acquisition of drug resistance by cancer cells are the major drawbacks in cancer therapy. A promising approach to overcome the posed barriers is conjugating tumor-homing peptides to drugs or nanocarriers. Such high-affinity peptides can specifically target surface markers overexpressed by cancer cells, ensuring a rapid and cancer-specific uptake of the drugs. Since prostate-specific membrane antigen (PSMA) is overexpressed by aggressive prostate cancer ...
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Purpose: Resistance to anticancer drugs is a serious obstacle to cancer chemotherapy. A common form of multidrug resistance (MDR) is caused by the overexpression of transmembrane transporter proteins P-glycoprotein (P-gp) and multidrug resistance-associated protein-1 (MRP1), encoded by MDR1 and MRP1 genes, respectively. These proteins lead to reduced intracellular drug concentration and decreased cytotoxicity by means of their ability to pump the drugs out of the cells. Breast cancer tumor resistance is mai...
Citation Formats
M. DEMİREL KARS, G. KARS, and U. Gündüz, “Paclitaxel Resistance in MCF-7/Pac Cell Line is Reversed Successfully by Saikosaponin A and Saikosaponin D,” UHOD-ULUSLARARASI HEMATOLOJI-ONKOLOJI DERGISI, pp. 227–232, 2013, Accessed: 00, 2020. [Online]. Available: