Mimicking corneal stroma using keratocyte-loaded photopolymerizable methacrylated gelatin hydrogels

Date

2018-04-01

Author

Bektas, Cemile Kilic
Hasırcı, Vasıf Nejat

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

232
views

0
downloads

Cell-laden methacrylated gelatin (GelMA) hydrogels with high (approximately 90%) transparency were prepared to mimic the natural form and function of corneal stroma. They were synthesized from GelMA with a methacrylation degree of 70% as determined by nuclear magnetic resonance. Hydrogels were strong enough to withstand handling. Stability studies showed that 87% of the GelMA hydrogels remained after 21 days in phosphate buffered saline (PBS). Cell viability in the first 2 days was over 90% for the human keratocytes loaded in the gels as determined with the live-dead analysis. Cells in the hydrogel elongated and connected to each other as observed by confocal laser scanning microscopy (CLSM) images and scanning electron microscope analysis after 3 weeks in the culture medium and cells were seen to be distributed throughout the hydrogel bulk. Cells were found to synthesize collagen Types I and V, decorin, and biglycan (representative collagens and proteoglycans of human corneal stroma, respectively) showing that keratocytes maintained their functions and preserved their phenotypes in the hydrogels. Transparency of cell-loaded and cell-free hydrogels after 21 days was found to be over 90% at all time points in the visible light range and was comparable to the transparency of the native cornea. The corneal stroma equivalent produced in this study that has cells entrapped in it leads to a product with homogenous distribution of cells. It was transparent at the very beginning and is expected to allow better vision than nontransparent substrates. It, therefore, has a significant potential to be used as an alternative to the current products used to treat corneal blindness.

Subject Keywords

Biomaterial, Corneal stroma substitute, GelMA, Hydrogels, Keratocytes, Photopolymerizable, Tissue engineering, Transparency

URI

https://hdl.handle.net/11511/31542

Journal

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE

DOI

https://doi.org/10.1002/term.2621

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Methacrylated gelatin hydrogels as corneal stroma substitutes: in vivo study BEKTAŞ, CEMİLE; Burcu, Ayse; Gedikoglu, Gokhan; Telek, Hande H.; Ornek, Firdevs; Hasırcı, Vasıf Nejat (2019-09-20) Methacrylated gelatin (GelMA) hydrogels were prepared to serve as corneal stroma equivalents. They were highly transparent (ca. 95% at 700 nm), mechanically strong and withstood handling and had high human corneal keratocyte viability (98%) after 21 days of culture period. In order to test the in vivo performance of the cell free GelMA hydrogels a pilot in vivo study was carried out using eyes of two white New Zealand rabbits. Hydrogel was implanted in a mid-stromal pocket created and without suture fixatio...
Contact guidance enhances the quality of a tissue engineered corneal stroma Vrana, E.; Builles, N.; Hindie, M.; Damour, O.; AYDINLI, ATİLLA; Hasırcı, Vasıf Nejat (2008-02-01) Corneal stroma is a very complex structure, composed of 200 lamellae of oriented collagen fibers. This highly complex nature of cornea is known to be important for its transparency and mechanical integrity. Thus, an artificial cornea design has to take into account this complex structure. In this study, behavior of human corneal keratocytes on collagen films patterned with parallel channels was investigated. Keratocytes proliferated well on films and reached confluency after 7 days in the incubation medium....
DEVELOPMENT OF 3D PRINTED PECTIN AND GELATIN BASED BIOACTIVE SKIN GRAFTS Bostancı, Nazlı Seray; Tezcaner, Ayşen; Hasırcı, Nesrin; Department of Biotechnology (2021-8-10) The aim of this study was to develop an easily applicable and bioactive skin graft composed of methacrylated pectin (PeMA) and methacrylated gelatin (GelMA), containing curcumin and/or Vitamin-C as antimicrobial and healing accelerating agents, respectively. Grafts were prepared in slab, 3D printed and 3D bioprinted forms and different properties were evaluated. Preparation parameters were optimized using slabs having different compositions (PeMA:GelMA as P:G 1:1, 1:2 and 1:3, v/v). P1:G1 solution did not h...
Experimental characterizations of the response of nematic liquid crystal droplets upon adsorption of nanoparticles from aqueous media Şengül, Selin; Büküşoğlu, Emre; Aydoğan, Nihal; Department of Chemical Engineering (2021-9-08) Nematic liquid crystal (LC) droplets have been widely used for the detection of molecular species. In this study, we investigated the response of micrometer sized nematic LC droplets against the adsorption of nanoparticles from aqueous media. In the first part of this study, we synthesized ~100 nm-in-diameter silica nanoparticles and modified their surfaces to mediate either planar or homeotropic LC anchoring and a pH-dependent charge. Secondly, we carried out the adsorption of nanoparticles to the LC-aqueo...
Nanofeatured silk fibroin membranes for dermal wound healing applications Karahaliloglu, Zeynep; Ercan, Batur; DENKBAŞ, EMİR BAKİ; Webster, Thomas J. (2015-01-01) As an effort to create the next generation of improved skin graft materials, in this study, we modified the surfaces of a previously investigated material, silk fibroin, using a NaOH alkaline treatment to obtain a biologically inspired nanofeatured surface morphology. Such surfaces were characterized for roughness, energy, and chemistry. In addition, keratinocyte (skin-forming cells) adhesion and proliferation on such nanofeatured silk fibroin wound dressings were studied in an initial attempt to determine ...

Citation Formats

C. K. Bektas and V. N. Hasırcı, “Mimicking corneal stroma using keratocyte-loaded photopolymerizable methacrylated gelatin hydrogels,” JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, pp. 0–0, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31542.