DEVELOPMENT OF 3D PRINTED PECTIN AND GELATIN BASED BIOACTIVE SKIN GRAFTS

2021-8-10
Bostancı, Nazlı Seray
The aim of this study was to develop an easily applicable and bioactive skin graft composed of methacrylated pectin (PeMA) and methacrylated gelatin (GelMA), containing curcumin and/or Vitamin-C as antimicrobial and healing accelerating agents, respectively. Grafts were prepared in slab, 3D printed and 3D bioprinted forms and different properties were evaluated. Preparation parameters were optimized using slabs having different compositions (PeMA:GelMA as P:G 1:1, 1:2 and 1:3, v/v). P1:G1 solution did not have proper viscosity and eliminated from printing studies. Based on the characterization studies, P1:G3 was chosen as the optimum composition. Curcumin (loaded as 100 or 150 μg/mL) release from the gels was higher at pH 7.4 than pH 5.0. The gels having higher curcumin demonstrated higher antibacterial effect on S.aureus but lower cell proliferation. For Vitamin-C (0.75 or 0.25 mM), spectroscopic detection was not succesful due to the interference of photo-crosslinker (Irgacure 2959). Therefore, the effect of Vitamin-C on collagen synthesis of fibroblasts was evaluated, where higher Vitamin-C containing gels generated higher collagen synthesis and enhanced cell proliferation. According to Live/Dead analyses, cell viability was above 80% for the samples having both bioactive agents upto 14 days. Bioprinted P1:G3 hydrogels containing L929 cells, curcumin and Vitamin-C resulted in high shape stability, viability and proliferation of the cells. The results indicated that curcumin and Vitamin-C containing P1:G3 hydrogels (either slab, printed or bioprinted) have high potential as skin graft and can be applied for the treatment of chronic wounds. In vivo studies are needed before any clinical application.

Suggestions

Fabrication and characterization of bilayered tissue scaffolds incorporating bioactive agents for skin tissue engineering applications
Aktürk, Ömer; Keskin, Dilek; Bilici, Temel; Department of Engineering Sciences (2015)
In this study, it was aimed to fabricate tissue scaffolds from different biological polymers (collagen, silk fibroin and sericin) for skin tissue engineering applications. For this purpose, bilayered scaffolds composed of epidermal (collagen/sericin films) and dermal (collagen sponges, collagen matrices or silk fibroin matrices) layers were produced with different biomaterial fabrication methods. Casting and solvent evaporation (film), lyophilization/freeze-drying (sponge) and dry/wet electro-spinning (micr...
Development of a calcium phosphate-gelatin composite as a bone substitute and its use in drug release
Yaylaoglu, MB; Korkusuz, P; Ors, U; Korkusuz, F; Hasırcı, Vasıf Nejat (1999-04-01)
This study was carried out to develop a calcium phosphate-gelatin composite implant that would mimic the structure and function of bone for use in filling voids or gaps and to release bioactive compounds like drugs, growth hormones into the implant site to assist healing. XDS analysis of the synthesized calcium phosphate revealed a calcium to phosphorus molar ratio of ca. 2.30, implying a less erodible material than hydroxyapatite (1.67). Release of the antibiotic gentamicin from the implant was with a burs...
pH responsive release of curcumin from photocrosslinked pectin/gelatin hydrogel wound dressings
Bostancı, Nazlı Seray; Büyüksungur, Senem; Hasırcı, Nesrin; Tezcaner, Ayşen (2022-01-01)
© 2022 Elsevier B.V.The aim of this study was to develop hydrogel wound dressings made of photocrosslinkable pectin and gelatin with pH dependent release of curcumin, an antimicrobial agent. Methacrylated forms of pectin and gelatin (PeMA and GelMA, respectively) were synthesized, and hydrogels were prepared with different compositions (1:1, 1:2 and 1:3 v/v ratios of PeMA and GelMA) by UV exposure. Pure GelMA was used as control group. Average pore diameter of hydrogels with the highest PeMA content (P1:G1)...
Mimicking corneal stroma using keratocyte-loaded photopolymerizable methacrylated gelatin hydrogels
Bektas, Cemile Kilic; Hasırcı, Vasıf Nejat (2018-04-01)
Cell-laden methacrylated gelatin (GelMA) hydrogels with high (approximately 90%) transparency were prepared to mimic the natural form and function of corneal stroma. They were synthesized from GelMA with a methacrylation degree of 70% as determined by nuclear magnetic resonance. Hydrogels were strong enough to withstand handling. Stability studies showed that 87% of the GelMA hydrogels remained after 21 days in phosphate buffered saline (PBS). Cell viability in the first 2 days was over 90% for the human ke...
Analysis of Antimicrobial and Coagulation Inducing Effects of a New Decontamination Powder
Tosun, Barış Çağrı; Öktem, Hüseyin Avni (2019-11-27)
In this study, the effect of a new formulation in the form of powder for the purpose of decontamination of skin from biological agents is investigated. Moreover, the formulation has also been shown to induce coagulation by erythrocyte aggregation, which is of significance since decontamination against B agents and bleeding wounds could be dealt with simultaneously by applying single formulation.
Citation Formats
N. S. Bostancı, “DEVELOPMENT OF 3D PRINTED PECTIN AND GELATIN BASED BIOACTIVE SKIN GRAFTS,” M.S. - Master of Science, Middle East Technical University, 2021.