Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization

Date

2016-09-01

Author

Koklukaya, Selva Zeynep
Sezer, Serdar
AKSOY, SERPİL
Hasırcı, Nesrin

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In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carrageenan (P(AAm-CA)/K-car) semi-interpenetrating network by entrapment method. Optimum pH and temperatures values were determined between 5.0-6.0 and 40-50 degrees C for free laccase (FL) and immobilized laccases, respectively. After 42 days of storage at 4 degrees C, FL and immobilized laccases retained their original activities in the range of 55%-73%. Percent decolorization of Acid Orange 52 by free enyzme and enyzmes immobilized in hydrogels was found between 63% and 39%. Percent decolorization of Acid Orange 52 in the presence of mediator by free enyzme was found 73% and enyzmes immobilized in hydrogels were found as 73%. (C) 2015 International Union of Biochemistry and Molecular Biology, Inc.

Subject Keywords

Semi-IPNs, Laccase, Immobilized enzyme, Decolorization, Acid Orange 52

URI

https://hdl.handle.net/11511/31922

Journal

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

DOI

https://doi.org/10.1002/bab.1417

Collections

Graduate School of Natural and Applied Sciences, Article

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Citation Formats

S. Z. Koklukaya, S. Sezer, S. AKSOY, and N. Hasırcı, “Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization,” BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, pp. 699–707, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31922.