Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization

Koklukaya, Selva Zeynep
Sezer, Serdar
Hasırcı, Nesrin
In this study, laccase from Trametes versicolor was immobilized in poly(acrylamide-crotonic acid)/sodium alginate [P(AAm-CrA)/alginate], poly(acrylamide-crotonic acid)/K-carrageenan [(P(AAm-CrA)/K-car)], poly(acrylamide-citraconic acid)/sodium alginate (P(AAm-CA)/alginate), and poly(acrylamide-citraconic acid)/K-carrageenan (P(AAm-CA)/K-car) semi-interpenetrating network by entrapment method. Optimum pH and temperatures values were determined between 5.0-6.0 and 40-50 degrees C for free laccase (FL) and immobilized laccases, respectively. After 42 days of storage at 4 degrees C, FL and immobilized laccases retained their original activities in the range of 55%-73%. Percent decolorization of Acid Orange 52 by free enyzme and enyzmes immobilized in hydrogels was found between 63% and 39%. Percent decolorization of Acid Orange 52 in the presence of mediator by free enyzme was found 73% and enyzmes immobilized in hydrogels were found as 73%. (C) 2015 International Union of Biochemistry and Molecular Biology, Inc.


Immobilization of laccase in kappa-carrageenan based semi-interpenetrating polymer networks
Makas, Y. Gizem; KALKAN, NİGAR; AKSOY, SERPİL; ALTINOK, HAYDAR; Hasırcı, Nesrin (2010-08-02)
Laccase enzyme (L) was immobilized by entrapment into semi-interpenetrating polymer networks (semi-IPNs) prepared from kappa-carrageenan with either poly(acrylamide-acrylic acid) [P(AAm-AA)/kappa-car] or poly(acrylamide-itaconic acid) [P(AAm-IA)/kappa-car]. For both systems, immobilized enzymes achieved the same optimum values observed for free enzyme (pH = 5.0 and T = 40 degrees C), except for P(AAm-IA)/kappa-car system there was a shift to 5.5 in optimum pH value. At the end of 42 days of storage immobili...
Semi-interpenetrating polymer networks (semi-IPNs) for entrapment of laccase and their use in Acid Orange 52 decolorization
Yamak, Ozgur; KALKAN, NİGAR; AKSOY, SERPİL; ALTINOK, HAYDAR; Hasırcı, Nesrin (2009-04-01)
Laccase enzyme (L) from Trametes versicolor was entrapped in three hydrogel structures namely poly(acrylamide-N-isopropylacrylamide), P(AAm-NIPA), and semi-interpenetrating networks of poly(-acrylamide)/alginate, P(AAm)/Alg, and poly(acrylamide-N-isopropylacrylamide)/alginate, P(AAm-NIPA)/Alg. The optimum temperatures for free and all immobilized systems were found to be 40 degrees C. For free and immobilized laccase systems of P(AAm-NIPA)-L, P(AAm)/Alg-L and P(AAm-NIPA)/Alg-L, K-m values were found to be 6...
DEMIRCIOGLU, H; BEYENAL, H; TANYOLAC, A; Hasırcı, Nesrin (1994-12-01)
Enzyme urease was immobilized in copolymer matrices of 2-hydroxyethyl methacrylate (HEMA) and N-vinyl pyrrolidone (VP). The activities of immobilized urease stored in phosphate buffer solution of pH 7.0 at 4-degrees-C were examined periodically for up to 90 days. For the matrices of higher VP/HEMA mole ratio in the structure, a higher volume increase and enhanced apparent activity were observed, while HEMA polymer alone proved to have the most stable matrix for prolonged activity. No appreciable amount o...
Electrorheological properties of kaolinite, polyindole, and polyindole/kaolinite composite suspensions
Arslan, Yasin; Unal, Halil Ibrahim; Yilmaz, Hasim; Sari, Bekir (Wiley, 2007-06-05)
In this study, polyindole (PIN) and polyindole/kaolinite (PIN/KAO) composite were synthesized by free radical polymerization using FeCl3 as an initiator. Average particle sizes (d(50)) of PIN and PIN/KAO composite were determined by dynamic light scattering (DLS) as 7.2 and 6.2 mu m, respectively. The samples were characterized by FTIR, elemental analysis, DSC/TGA and SEM measurements. Suspensions of KAO, PIN, and PIN/KAO composite were prepared in silicone oil (SO) and the sedimentation stabilities were de...
Stereocontrolled 1,3-dipolar cycloadditions using Oppolzer's camphor sultam as the chiral auxiliary for carbonyl stabilized azomethine ylides
Garner, Philip; Doğan, Özdemir; Youngs, Wiley; Vance, Kennedy; Protasiewicz, John; Zaniewski, Rebecca (2001-01-01)
Two complementary approaches to substituted pyrrolidines via stereocontrolled 1,3-dipolar cycloaddition reactions of chiral azomethine ylides are described. In one approach, chiral azomethine ylides were generated by thermolysis of aziridine carboxylate sultams and trapped with a variety of dipolarophiles to give good yields of the corresponding cycloadducts. In the second approach, chiral azomethine ylides were generated from glycyl sultams by 'imine tautomerization' and trapped with dipolarophiles to give...
Citation Formats
S. Z. Koklukaya, S. Sezer, S. AKSOY, and N. Hasırcı, “Polyacrylamide-based semi-interpenetrating networks for entrapment of laccase and their use in azo dye decolorization,” BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, pp. 699–707, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/31922.