Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Modelling of long-term photoresponse of bacteriorhodopsin immobilized on cellulose acetate membranes
Date
1996-05-01
Author
Sediroglu, V
Aydemir, A
Gündüz, Ufuk
Yucel, M
Turker, L
Eroglu, I
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
182
views
0
downloads
Cite This
Purple membrane fragments with its integrated protein bacteriorhodopsin were immobilized on lipid impregnated and lipid free cellulose acetate membranes. Two mathematical models were suggested to simulate the photoresponse curves of these membranes which were obtained by measuring the pH variation independently in each chamber of a specifically designed photoactivity cell for long periods of illumination. The first order model suggested was mainly based on the existence of light phase deprotonation and dark phase reprotonation reactions. The overall deprotanation rate constants were found to be slightly less than the overall reprotonation rate constants with the first order kinetics. In the second model, the pH change-time data were expressed by a mathematical decay function. There has been found a great agreement between the first order rate constants and the decay constants.
Subject Keywords
Bacteriorhodopsin
,
Halobacterium halobium
,
Intelligent material
,
Biosynthetic membrane
,
Photoresponse model
URI
https://hdl.handle.net/11511/32043
Journal
JOURNAL OF MEMBRANE SCIENCE
DOI
https://doi.org/10.1016/0376-7388(95)00216-2
Collections
Graduate School of Natural and Applied Sciences, Article
Suggestions
OpenMETU
Core
MODELING AND KINETICS OF LIGHT-INDUCED PROTON PUMPING OF BACTERIORHODOPSIN RECONSTITUTED LIPOSOMES
EROGLU, I; ZUBAT, BM; Yücel, Ayşe Meral (1991-09-01)
Purple membrane fragments isolated from the cell membrane of the photosynthetic bacteria Halobacterium halobium S.9 strain are incorporated into egg yolk phosphatidylcholine liposomes. Purple membrane contains crystalline patches of a retinal protein called bacteriorhodopsin. Upon illumination, bacteriorhodopsin undergoes a reversible photoreaction in which a proton is released on one side of the membrane and a proton is bound on the other side, thus resulting in an electro-chemical gradient across the me...
Comparison of antioxidant enzyme activities and DNA damage in chickpea (Cicer arietinum L.) genotypes exposed to vanadium
Imtiaz, Muhammad; Mushtaq, Muhammad Adnan; Rizwan, Muhammad Shahid; Arif, Muhammad Saleem; Yousaf, Balal; Ashraf, Muhammad; Xiong Shuanglian, Xiong Shuanglian; Rizwan, Muhammad; Mehmood, Sajid; Tu, Shuxin (2016-10-01)
The present study was done to elucidate the effects of vanadium (V) on photosynthetic pigments, membrane damage, antioxidant enzymes, protein, and deoxyribonucleic acid (DNA) integrity in the following chickpea genotypes: C-44 (tolerant) and Balkasar (sensitive). Changes in these parameters were strikingly dependent on levels of V, at 60 and 120 mg V L-1 induced DNA damage in Balkasar only, while photosynthetic pigments and protein were decreased from 15 to 120 mg V L-1 and membrane was also damaged. It was...
Investigation of the effect of GPCR oligomerization on the GNAi1 protein homodimerization in live cells using FRET
Nalli, Enise; Son, Çağdaş Devrim; Küçük Baloğlu, Fatma; Department of Biotechnology (2022-1-26)
G-Protein Coupled Receptors (GPCR) are membrane proteins that pass the cell membrane seven times. In classical GPCR signaling pathways, one GPCR-one heterotrimeric G-protein interaction model is enough to transmit the signal to effector proteins. Studies since 2000 showed that one GPCR dimer-one heterotrimeric G-protein interaction model is more likely, and GPCRs having homo- /hetero- dimers interact with a single G⍺-protein. Recently, studies on GPCRs indicated that more than two receptors interact to ...
Evaluation of functional changes in akr1b1 and akr1b10 overexpressing colorectal cancer cell lines
Güderer, İsmail; Banerjee, Sreeparna; Department of Biology (2021-2-15)
Aldo-keto reductases (AKRs) are nicotinamide adenine dinucleotide phosphate (NADPH)-dependent enzymes with diverse cellular metabolism functions. AKR1B1 and AKR1B10 are two of the most studied enzymes in the AKR family. AKR1B1 reduces excess glucose into sorbitol using reducing electrons from NADPH, and the hyperactivation of the AKR1B1 pathways is associated with oxidative stress and cell death. AKR1B10 is a poor reductant of glucose but is a vital enzyme that can metabolize retinol and many other drugs an...
Investigation of phase inversion behavior of cellulose- ionic liquid solutions in relationship with membrane formation
Durmaz, Elif Nur; Çulfaz Emecen, Pınar Zeynep; Department of Chemical Engineering (2017)
Cellulose membranes were produced from ionic liquid solutions by phase inversion technique and thermodynamic and kinetic aspects of the process were investigated to relate these to membrane morphology and performance. In thermodynamics part, polymer-solvent, polymer-nonsolvent and polymer-solvent-nonsolvent interactions were examined experimentally, together with Hansen solubility parameter estimations. Kinetics part consisted of measuring phase inversion rate. Obtained membranes were characterized by their...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
V. Sediroglu, A. Aydemir, U. Gündüz, M. Yucel, L. Turker, and I. Eroglu, “Modelling of long-term photoresponse of bacteriorhodopsin immobilized on cellulose acetate membranes,”
JOURNAL OF MEMBRANE SCIENCE
, pp. 65–71, 1996, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32043.