The role of multiple toll-like receptor signalling cascades on interactions between biomedical polymers and dendritic cells

2010-08-01
Shokouhi, Behnaz
Coban, Cevayir
Hasırcı, Vasıf Nejat
Aydin, Erkin
Dhanasingh, Anandhan
Shi, Nian
Koyama, Shohei
Akira, Shizuo
Zenke, Martin
Sechi, Antonio S.
Biomaterials are used in several health-related applications ranging from tissue regeneration to antigen-delivery systems. Yet, biomaterials often cause inflammatory reactions suggesting that they profoundly alter the homeostasis of host immune cells such as dendritic cells (DCs). Thus, there is a major need to understand how biomaterials affect the function of these cells. In this study, we have analysed the influence of chemically and physically diverse biomaterials on DCs using several murine knockouts. DCs can sense biomedical polymers through a mechanism, which involves multiple TLR/MyD88-dependent signalling pathways, in particular TLR2. TLR4 and TLR6. TLR biomaterial interactions induce the expression of activation markers and pro-inflammatory cytokines and are sufficient to confer on DCs the ability to activate antigen-specific T cells. This happens through a direct biomaterial DC interaction although, for degradable biomaterials, soluble polymer molecules can also alter DC function. Finally, the engagement of TLRs by biomaterials profoundly alters DC adhesive properties. Our findings could be useful for designing structure function studies aimed at developing more bioinert materials. Moreover, they could also be exploited to generate biomaterials for studying the molecular mechanisms of TLR signalling and DC activation aiming at fine-tuning desired and pre-determined immune responses.

Suggestions

The influence of elastin-like recombinant polymer on the self-renewing potential of a 3D tissue equivalent derived from human lamina propria fibroblasts and oral epithelial cells
Kinikoglu, Beste; Carlos Rodriguez-Cabello, Jose; Damour, Odile; Hasırcı, Vasıf Nejat (2011-09-01)
Three-dimensional epithelial tissue equivalents tend to lose their self-renewing potential progressively during culture as their epithelial cells lose their proliferative capacity with time. Even though the tissue engineered construct can mimic the native tissue well, it rapidly degrades after implantation due to the insufficient number of proliferating cells in the equivalent. In the present study we demonstrate for the first time that the use of an elastin-like recombinant polymer (ELR) engineered to cont...
Role of Vibrational Spectroscopy in Stem Cell Research
Aksoy, Ceren; Severcan, Feride (2012-01-01)
Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the ste...
Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments
Kalyoncuoglu, Ulku Tugba; Yilmaz, Bengi; Koc, Serap Gungor; Evis, Zafer; ARPACI, PEMBEGÜL UYAR; Kansu, Gulay (Wiley, 2018-12-01)
Background: For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms. Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2O3) and zirconia (ZrO2) implant systems are widely used in the area. However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.
The Behaviour of Neuro-2A Cells on Silicon Substrates with Various Topographies Generated by Femtosecond Laser Micromachining
Mingu, Sara; Pavlov, Ihor; Son, Çağdaş; Bek, Alpan (SCITEPRESS; 2020-2-24)
The interaction of neural cells with silicon surfaces is important for basic research as well as for various possible applications, such as silicon-based neural implants and neurochips. Laser structuring of silicon provides a quick and versatile method for the generation of complex, hierarchical topographies on precise locations of the substrate. The behaviour ofNeuro-2A cells with laser-structured silicon substrates was studied using a live-imaging setup with fluorescence microscopy. Neuro-2A cells were ab...
Differential immune activation following encapsulation of immunostimulatory CpG oligodeoxynucleotide in nanoliposomes.
Erikçi, E; Gürsel, Mayda; Gürsel, I (2011-02-01)
The immunogenicity of a vaccine formulation is closely related to the effective internalization by the innate immune cells that provide prolonged and simultaneous delivery of antigen and adjuvant to relevant antigen presenting cells. Endosome associated TLR9 recognizes microbial unmethylated CpG DNA. Clinical applications of TLR9 ligands are significantly hampered due to their pre-mature in vivo digestion and rapid clearance. Liposome encapsulation is a powerful tool to increase in vivo stability as well as...
Citation Formats
B. Shokouhi et al., “The role of multiple toll-like receptor signalling cascades on interactions between biomedical polymers and dendritic cells,” BIOMATERIALS, pp. 5759–5771, 2010, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32069.