Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Role of Vibrational Spectroscopy in Stem Cell Research
Download
index.pdf
Date
2012-01-01
Author
Aksoy, Ceren
Severcan, Feride
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
195
views
58
downloads
Cite This
Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the stem cells have some unwanted effects on the properties of stem cells, and these methods also do not provide real-time information about cellular conditions. These challenges enforce the usage of nondestructive, rapid, sensitive, high quality, label-free, cheep, and innovative chemical monitoring methods. In this context, vibrational spectroscopy provides promissing alternative to get new information into the field of stem cell biology for chemical analysis, quantification, and imaging of stem cells. Raman and infrared spectroscopy and imaging can be used as a new complimentary spectroscopic approaches to gain new insight into stem cell reseaches for future therapeutic and regenerative medicines. In this paper, recent developments in applications of vibrational spectroscopy techniques for stem cell characterization and identification are presented.
Subject Keywords
Raman spectroscopy
,
Raman microspectroscopy
,
Infrared spectroscopy
,
Infrared microspectroscopy
,
Embryonic stem cells
,
Adult stem cells
,
Mesenchymal stem cells
URI
https://hdl.handle.net/11511/51537
Journal
SPECTROSCOPY-AN INTERNATIONAL JOURNAL
DOI
https://doi.org/10.1155/2012/513286
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
Characterization and identification of human mesenchymal stem cells at molecular level
Aksoy, Ceren; Severcan, Feride; Çetinkaya, Duygu Uçkan; Department of Biotechnology (2012)
Bone marrow mesenchymal stem cells (BM-MSCs) are pluripotent cells that can differentiate into a variety of non-hematopoietic tissues. They also maintain healthy heamatopoiesis by providing supportive cellular microenvironment into BM. In this thesis, MSCs were characterized in terms of their morphological, immunophenotypical and differentiation properties. Then, they were examined by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy together with hierarchical clustering, and FT...
Influence of micropatterns on human mesenchymal stem cell fate /
Hastürk, Onur; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin; Department of Biotechnology (2016)
Mesenchymal stem cells (MSCs) are promising cell sources for tissue engineering applications as they can differentiate into a variety of adult cells types including osteoblasts. In vivo microenvironment of stem cells is known to provide both biochemical signals and micro- and nanoscale physical cues that influence the behavior and fate of stem cells. The use of soluble chemical factors is the most common strategy to guide the commitment of MSCs to specific lineages, but it is a cause of concern such as unsa...
Influence of microenvironment on tissue engineering applications
Sayın, Esen; Hasırcı, Vasıf Nejat; Baran, Erkan Türker; Department of Biotechnology (2017)
Cues of microenvironment that guide both mature and stem cells determine the success of tissue engineered constructs. To prove and emphasize this expectation, various parameters such as surface topography, scaffold (cell carrier, scaffold) chemistry, 2D vs 3D microenvironments and mechanical stimulation were included into the microenvironment. Surfaces with two distinct physical cues pillar and groove-ridge type micropatterns were transferred to the surfaces of the films by casting the collagen type I and s...
Evaluating Oxygen Tensions Related to Bone Marrow and Matrix for MSC Differentiation in 2D and 3D Biomimetic Lamellar Scaffolds
Sayin, Esen; Baran, Erkan Turker; Elsheikh, Ahmed; Mudera, Vivek; Cheema, Umber; Hasırcı, Vasıf Nejat (2021-04-01)
The physiological O-2 microenvironment of mesenchymal stem cells (MSCs) and osteoblasts and the dimensionality of a substrate are known to be important in regulating cell phenotype and function. By providing the physiologically normoxic environments of bone marrow (5%) and matrix (12%), we assessed their potential to maintain stemness, induce osteogenic differentiation, and enhance the material properties in the micropatterned collagen/silk fibroin scaffolds that were produced in 2D or 3D. Expression of ost...
Arid4b alters cell cycle and cell death dynamics during mouse embryonic stem cell differentiation.
Güven, Gözde; Terzi Çizmecioğlu, Nihal (2021-02-09)
Cell division and death play an important role in embryonic development. Cell specialization is accompanied with slow proliferation and quiescence. Cell death is important for morphogenesis. Gene expression changes during differentiation is coordinated by lineage-specific transcription factors and chromatin factors. It is not yet fully understood how alterations in gene expression and cell cycle/death mechanisms are connected. We previously identified a chromatin protein Arid4b as a critical factor for meso...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
C. Aksoy and F. Severcan, “Role of Vibrational Spectroscopy in Stem Cell Research,”
SPECTROSCOPY-AN INTERNATIONAL JOURNAL
, pp. 167–184, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51537.