PHOTORESPONSE OF BACTERIORHODOPSIN IMMOBILIZED IN POLYACRYLAMIDE-GEL MEMBRANES

Date

1994-01-27

Author

EROGLU, I
AYDEMIR, A
TURKER, L
Yücel, Ayşe Meral

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A two-chambered cell has been designed and constructed for the measurement of photoresponses of bacteriorhodopsin immobilized in polyacrylamide gel (FAG) membranes. The photoresponse of bacteriorhodopsin was determined by measuring the pH variation in each chamber independently, for long periods of illumination. The aim was to verify the light driven proton transfer of bacteriorhodopsin immobilized in FAG membranes. However, the same pH variation was observed in the two chambers irrespective of whether oriented or nonoriented bacteriorhodopsin immobilized in FAG membranes were employed.

Subject Keywords

Bacteriorhodopsin, Purple membrane, Polyacrylamide gel, Photoresponse, Proton pump

URI

https://hdl.handle.net/11511/32586

Collections

Graduate School of Natural and Applied Sciences, Article