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PHOTORESPONSE OF BACTERIORHODOPSIN IMMOBILIZED IN POLYACRYLAMIDE-GEL MEMBRANES
Date
1994-01-27
Author
EROGLU, I
AYDEMIR, A
TURKER, L
Yücel, Ayşe Meral
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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A two-chambered cell has been designed and constructed for the measurement of photoresponses of bacteriorhodopsin immobilized in polyacrylamide gel (FAG) membranes. The photoresponse of bacteriorhodopsin was determined by measuring the pH variation in each chamber independently, for long periods of illumination. The aim was to verify the light driven proton transfer of bacteriorhodopsin immobilized in FAG membranes. However, the same pH variation was observed in the two chambers irrespective of whether oriented or nonoriented bacteriorhodopsin immobilized in FAG membranes were employed.
Subject Keywords
Bacteriorhodopsin
,
Purple membrane
,
Polyacrylamide gel
,
Photoresponse
,
Proton pump
URI
https://hdl.handle.net/11511/32586
Journal
JOURNAL OF MEMBRANE SCIENCE
DOI
https://doi.org/10.1016/0376-7388(93)e0138-a
Collections
Graduate School of Natural and Applied Sciences, Article