PHOTORESPONSE OF BACTERIORHODOPSIN IMMOBILIZED IN POLYACRYLAMIDE-GEL MEMBRANES

Date

1994-01-27

Author

EROGLU, I
AYDEMIR, A
TURKER, L
Yücel, Ayşe Meral

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

52
views

0
downloads

A two-chambered cell has been designed and constructed for the measurement of photoresponses of bacteriorhodopsin immobilized in polyacrylamide gel (FAG) membranes. The photoresponse of bacteriorhodopsin was determined by measuring the pH variation in each chamber independently, for long periods of illumination. The aim was to verify the light driven proton transfer of bacteriorhodopsin immobilized in FAG membranes. However, the same pH variation was observed in the two chambers irrespective of whether oriented or nonoriented bacteriorhodopsin immobilized in FAG membranes were employed.

Subject Keywords

Bacteriorhodopsin, Purple membrane, Polyacrylamide gel, Photoresponse, Proton pump

URI

https://hdl.handle.net/11511/32586

Journal

JOURNAL OF MEMBRANE SCIENCE

DOI

https://doi.org/10.1016/0376-7388(93)e0138-a

Collections

Graduate School of Natural and Applied Sciences, Article

Suggestions

OpenMETU
Core

Development of self-humidifying nano-composite membrane for polymer electrolyte membrane fuel cell Çaçan, Umut Baki; Özkan, Necati; Devrim, Yılser; Department of Polymer Science and Technology (2015) Low humidity self-humidifying nano-composite membrane electrode assemblies (MEA) were developed for Polymer Electrolyte Membrane Fuel Cell (PEMFC) working at elevated temperatures. The nano-composite membranes were prepared by adding nano-sized silica particles (SiO2) or inorganic fillers with a size of approximately 20 nm to a polymeric material which is commercially named as Nafion (Perfluoro Sulfonic Acid/PFSA). The particle content of the nano-composite membranes were between 2.5 – 10 wt. %. In this man...
Modelling of long-term photoresponse of bacteriorhodopsin immobilized on cellulose acetate membranes Sediroglu, V; Aydemir, A; Gündüz, Ufuk; Yucel, M; Turker, L; Eroglu, I (1996-05-01) Purple membrane fragments with its integrated protein bacteriorhodopsin were immobilized on lipid impregnated and lipid free cellulose acetate membranes. Two mathematical models were suggested to simulate the photoresponse curves of these membranes which were obtained by measuring the pH variation independently in each chamber of a specifically designed photoactivity cell for long periods of illumination. The first order model suggested was mainly based on the existence of light phase deprotonation and dark...
Photoelectrochemical H-2 production by using bacteriorhodopsin immobilized in PAG Yucel, M; El-Bashiti, T; Eroğlu, İnci; Sediroglu, V; Turker, L; Gündüz, Ufuk (2000-06-15) Bacteriorhodopsin (BR), a protein found in the purple membrane of Halobacterium halobium is used as a biocatalyst in photoelectrochemical hydrogen production in a NaCl solution. In present study the biocatalytic effect of BR of a new strain MILTU and strain S-9 were investigated in immobilized membranes The photoelectrochemical hydrogen production experiments were performed in a batch type glass reactor with free H. halobium packed cells or H halobium cells immobilized in polyacrylamide gel (PAG). It was fo...
GAS PERMEATION CHARACTERISTICS OF POLYMER-ZEOLITE MIXED MATRIX MEMBRANES SUER, MG; BAC, N; Yılmaz, Levent (1994-05-20) Mixed matrix membranes of polyethersulfone (PES), a glassy polymer, and hydrophilic zeolites 13X and 4A were prepared by using different membrane preparation procedures. Using selected procedure (c), the permeation rates of N2, O2, Ar, CO2 and H-2 were measured with a variety of membranes prepared at different zeolite loadings. Significant differences in measured permeability and calculated selectivity values demonstrated the importance of the type and percentage of zeolite. For both zeolitic additives, per...
Covalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeads ALTINOK, HAYDAR; AKSOY, SERPİL; TÜMTÜRK, HAYRETTİN; Hasırcı, Nesrin (2008-06-01) A carrier for invertase enzyme was synthesized from styrene (S) and 2- hydroxyethyl methacrylate (HEMA) in the form of microbeads. These poly (styrene-2-hydroxyethyl methacrylate), P(S-HEMA) microbeads were activated by epichlorohydrin (ECH) treatment for covalent immobilization. The free and immobilized invertase were assayed in the hydrolysis of sucrose to glucose, and the obtained results were compared. The optimum pH was 4.5 for free and 5.5 for immobilized invertase. The optimum temperature of invertas...

Citation Formats

I. EROGLU, A. AYDEMIR, L. TURKER, and A. M. Yücel, “PHOTORESPONSE OF BACTERIORHODOPSIN IMMOBILIZED IN POLYACRYLAMIDE-GEL MEMBRANES,” JOURNAL OF MEMBRANE SCIENCE, pp. 171–179, 1994, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32586.