Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Covalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeads
Date
2008-06-01
Author
ALTINOK, HAYDAR
AKSOY, SERPİL
TÜMTÜRK, HAYRETTİN
Hasırcı, Nesrin
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
197
views
0
downloads
Cite This
A carrier for invertase enzyme was synthesized from styrene (S) and 2- hydroxyethyl methacrylate (HEMA) in the form of microbeads. These poly (styrene-2-hydroxyethyl methacrylate), P(S-HEMA) microbeads were activated by epichlorohydrin (ECH) treatment for covalent immobilization. The free and immobilized invertase were assayed in the hydrolysis of sucrose to glucose, and the obtained results were compared. The optimum pH was 4.5 for free and 5.5 for immobilized invertase. The optimum temperature of invertase shifted from 45C to 55C upon immobilization. For free and immobilized enzymes, kinetic parameters were calculated as 4.1 x 10(-3) mol L(-1)and 9.2 x 10(-3) mol L(-1)for K-m, and 6.6 x 10(-2) mol L-1 min(-1)and 4.1 x 10(-1) mol L-1 min(-1)for V-max, respectively. After 1 month of storage at 4C, free enzyme retained 36% of its initial activity, while for the ECH-activated P(S-HEMA) immobilized enzyme, P(S-HEMA)-E, this value was observed as 67%. In repeated batch use, i.e., 20 times in 3 days, 78% retention of the initial activity was observed for P(S-HEMA)-E system.
Subject Keywords
Copolymer Matrices
,
Graft-Copolymers
,
Acid) Hydrogels
,
Enzyme-Activity
,
Alpha-Amylase
,
Ca2+ Ions
,
Gel Beads
,
Hydrolysis
,
Membrane
,
Urease
URI
https://hdl.handle.net/11511/32498
Journal
JOURNAL OF FOOD BIOCHEMISTRY
DOI
https://doi.org/10.1111/j.1745-4514.2008.00156.x
Collections
Graduate School of Natural and Applied Sciences, Article
Suggestions
OpenMETU
Core
Enzymatically induced mineralization of platelet-rich fibrin
Douglas, Timothy E. L.; Gassling, Volker; Declercq, Heidi A.; Purcz, Nicolai; Pamula, Elzbieta; Haugen, Havard J.; Chasan, Safak; de Mulder, Eric L. W.; Jansen, John A.; Leeuwenburgh, Sander C. G. (Wiley, 2012-05-01)
Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with calcium phosphate (CaP) to improve PRFs suitability as a material for bone replacement. Incorporated ALP retained its bioactivity and induced formation of CaP material within PRF membranes, as confirmed by SEM, ...
Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry
BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2015-06-13)
Anion and cation exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS) were used for speciation of selenium in supplements. All the parameters in the extraction, separation, and determination procedures were optimized. Recovery studies for the selenium species from the anion and cation exchange columns were performed and there were no analyte losses. Limits of detection for selenium(IV), selenium(VI), Se in selenomethionine, and Se in sele...
Covalent immobilization of chloroperoxidase onto magnetic beads: Catalytic properties and stability
Bayramoglu, Guelay; Kiralp, Senem; Yilmaz, Meltem; Toppare, Levent Kamil; Arica, M. Yakup (Elsevier BV, 2008-02-15)
Amino groups containing magnetic beads were used in covalent immobilization of the enzyme "chloroperoxidase (CPO)" which is one of a few enzymes that can catalyse the peroxide dependent oxidation of a wide spectrum of organic and inorganic compounds. The magnetic poly(glycidylmethacrylate-methylmethacrylate-etbyleneglycol dimethacrylate), magnetic p(GMA-MMA-EGDMA) beads were prepared via suspension polymerization in the presence of ferric ions. The magnetic beads were characterized with scanning electron mi...
Speciation of Arsenic in Fish by High-Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry
Ozcan, Sefika; BAKIRDERE, Sezgin; Ataman, Osman Yavuz (2016-01-01)
Arsenic speciation in fish was performed using isocratic elution with cation exchange column high-performance liquid chromatography and inductively coupled plasma-mass spectrometry detection. The separation of arsenobetaine and dimethylarsinic acid was performed in 12 min. DOLT-4 dogfish liver and NIST RM-50 albacore tuna certified reference materials were used to evaluate the accuracy for the determination of total arsenic in fish. In addition, reference materials were analyzed for arsenobetaine and dimeth...
Novel poly(2,5-dithienylpyrrole) (PSNS) derivatives functionalized with azobenzene, coumarin and fluorescein chromophore units: spectroelectrochemical properties and electrochromic device applications
Yigit, Deniz; Hacioglu, Serife O.; GÜLLÜ, Mustafa; Toppare, Levent Kamil (2015-01-01)
Three novel 2,5-dithienylpyrrole (SNS) derivatives containing strong chromophore units such as azobenzene, coumarin and fluorescein were synthesized to investigate the effects of chromophore substituents on the electrochemical and spectroelectrochemical properties of resulting polymers. Electrochemical and optoelectronic characteristics of the homopolymers, poly(1-(2-(4-(phenyldiazenyl)phenoxy)ethyl)-2,5di(thiophen-2-yl)-1H-pyrrole) (PTPTAz), poly(4-(2-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)ethoxy)-2H-chrome...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
H. ALTINOK, S. AKSOY, H. TÜMTÜRK, and N. Hasırcı, “Covalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeads,”
JOURNAL OF FOOD BIOCHEMISTRY
, pp. 299–315, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32498.