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Covalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeads
Date
2008-06-01
Author
ALTINOK, HAYDAR
AKSOY, SERPİL
TÜMTÜRK, HAYRETTİN
Hasırcı, Nesrin
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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A carrier for invertase enzyme was synthesized from styrene (S) and 2- hydroxyethyl methacrylate (HEMA) in the form of microbeads. These poly (styrene-2-hydroxyethyl methacrylate), P(S-HEMA) microbeads were activated by epichlorohydrin (ECH) treatment for covalent immobilization. The free and immobilized invertase were assayed in the hydrolysis of sucrose to glucose, and the obtained results were compared. The optimum pH was 4.5 for free and 5.5 for immobilized invertase. The optimum temperature of invertase shifted from 45C to 55C upon immobilization. For free and immobilized enzymes, kinetic parameters were calculated as 4.1 x 10(-3) mol L(-1)and 9.2 x 10(-3) mol L(-1)for K-m, and 6.6 x 10(-2) mol L-1 min(-1)and 4.1 x 10(-1) mol L-1 min(-1)for V-max, respectively. After 1 month of storage at 4C, free enzyme retained 36% of its initial activity, while for the ECH-activated P(S-HEMA) immobilized enzyme, P(S-HEMA)-E, this value was observed as 67%. In repeated batch use, i.e., 20 times in 3 days, 78% retention of the initial activity was observed for P(S-HEMA)-E system.
Subject Keywords
Copolymer Matrices
,
Graft-Copolymers
,
Acid) Hydrogels
,
Enzyme-Activity
,
Alpha-Amylase
,
Ca2+ Ions
,
Gel Beads
,
Hydrolysis
,
Membrane
,
Urease
URI
https://hdl.handle.net/11511/32498
Journal
JOURNAL OF FOOD BIOCHEMISTRY
DOI
https://doi.org/10.1111/j.1745-4514.2008.00156.x
Collections
Graduate School of Natural and Applied Sciences, Article