Photoelectrochemical H-2 production by using bacteriorhodopsin immobilized in PAG

Date

2000-06-15

Author

Yucel, M
El-Bashiti, T
Eroğlu, İnci
Sediroglu, V
Turker, L
Gündüz, Ufuk

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Bacteriorhodopsin (BR), a protein found in the purple membrane of Halobacterium halobium is used as a biocatalyst in photoelectrochemical hydrogen production in a NaCl solution. In present study the biocatalytic effect of BR of a new strain MILTU and strain S-9 were investigated in immobilized membranes The photoelectrochemical hydrogen production experiments were performed in a batch type glass reactor with free H. halobium packed cells or H halobium cells immobilized in polyacrylamide gel (PAG). It was found that the presence of BR in the reactor enhances the hydrogen production rate upon illumination. Immobilization increased the amount of hydrogen produced per mole of BR. The maximum moles of hydrogen produced per mote of BR used was obtained by H. halobium MILTU packed cells immobilized in PAG. The presence of BR regulates the pH of the system, increases the hydrogen production rate and causes light-induced proton dissociation, which lowers the electrical power requirement for the electrochemical conversion.

Subject Keywords

Biological hydrogen, Electrochemistry, Hydrogen production, Membrane, Photoelectrochemistry

URI

https://hdl.handle.net/11511/55952

Conference Name

13th World Hydrogen Energy Conference

Collections

Graduate School of Natural and Applied Sciences, Conference / Seminar

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Citation Formats

M. Yucel, T. El-Bashiti, İ. Eroğlu, V. Sediroglu, L. Turker, and U. Gündüz, “Photoelectrochemical H-2 production by using bacteriorhodopsin immobilized in PAG,” presented at the 13th World Hydrogen Energy Conference, BEIJING, PEOPLES R CHINA, 2000, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55952.