Model based dynamics analysis in live cell microtubule images.

Date

2007-07-10

Author

Altinok, A
Kiriş, Erkan
Peck, AJ
Feinstein, SC
Wilson, L
Manjunath, BS
Rose, K

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Background: The dynamic growing and shortening behaviors of microtubules are central to the fundamental roles played by microtubules in essentially all eukaryotic cells. Traditionally, microtubule behavior is quantified by manually tracking individual microtubules in time-lapse images under various experimental conditions. Manual analysis is laborious, approximate, and often offers limited analytical capability in extracting potentially valuable information from the data. Results: In this work, we present computer vision and machine-learning based methods for extracting novel dynamics information from time-lapse images. Using actual microtubule data, we estimate statistical models of microtubule behavior that are highly effective in identifying common and distinct characteristics of microtubule dynamic behavior. Conclusion: Computational methods provide powerful analytical capabilities in addition to traditional analysis methods for studying microtubule dynamic behavior. Novel capabilities, such as building and querying microtubule image databases, are introduced to quantify and analyze microtubule dynamic behavior.

Subject Keywords

Hide Markov model, Behavior pattern, Live cell image, Observation sequence, Path cover

URI

https://hdl.handle.net/11511/33356

Journal

BMC cell biology

DOI

https://doi.org/10.1186/1471-2121-8-s1-s4

Collections

Department of Biology, Article

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Citation Formats

A. Altinok et al., “Model based dynamics analysis in live cell microtubule images.,” BMC cell biology, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/33356.