Vacuum infiltration based agrobacterium mediated gene transfer to lentil (Lens culinaris M.) tissues

Mahmoudian, M
Celikkol, U
Yucel, M
Öktem, Hüseyin Avni
A highly efficient Agrobacterium-mediated transformation system has been developed for lentils (Lens culinaris M.) using vacuum infiltration of cotyledonary nodes and nodal segments with Agrobacterium suspension. This procedure exhibits distinct advantages over those previously reported in lentils, in that it uses vacuum infiltration enhancive force to introduce Agrobacterium suspension into the highly regenerable cotyledonary node meristems or nodal segments, which in turn rapidly produce transgenic shoots without an intermediate callus phase. The efficiency of the system has been investigated using GUS histochemical assays and PCR amplification which were evidencing integration of the transgenes and superiority of this system over conventional Agrobacterium mediated procedures that has been so far applied for lentil transformation.


Laser induced periodic surface structuring for surface enhanced raman spectroscopy
Özkarslıgil, Zeynep Tuğçe; Bek, Alpan; Department of Physics (2020)
In this study, our aim is to fabricate and characterize efficient substrates of surface enhanced Raman spectroscopy (SERS) utilizing the field enhancement due to hot spots made by recently developed method of laser induced periodic surface structuring (LIPSS). LIPSS is a cost-effective technique for rapid processing of almost any materials compared to conventional lithography methods. Coating of a thin silver film on LIPSS applied substrate surface provides to observe the localized surface plasmon effect fo...
Oz, M. T.; EYİDOĞAN, FÜSUN; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (2009-08-01)
An efficient selection system and Agrobacterium mediated transformation for chickpea (Cicer arietinum L.) cotyledonary nodes were investigated. Effect of selective agents and antibiotics on multiple shoot and root induction of cotyledonary nodes and effects of mechanical injury and vacuum infiltration on transformation efficiency were evaluated. Selective agents and antibiotics were applied to explants at different concentrations for one month and numbers of regenerated shoots and roots were recorded. Kanam...
Optimization of Selective Electrophoretic Deposition and Isostatic Compression of Titania Nanoparticles for Flexible Dye-Sensitized Solar Cells
Kocaoglu, Bahadir Can; Icli, Kerem Cagatay; Özenbaş, Ahmet Macit (2016-04-01)
Flexible photoanodes on PEN/PET based substrates for dye-sensitized solar cells (DSSC) by using electrophoretic deposition (EPD) method for the formation of titania nanoporous layer with titania nanoparticles (P-25) were produced. Considering commercially available titania nanoparticles containing both anatase and rutile phases, the undesired rutile nanoparticles, for DSSC applications, were almost eliminated from the coatings by selective pH adjustment of the EPD solutions without any binders. The coating ...
Self-Assembled ZnO Nanosheet-Based Spherical Structure as Photoanode in Dye-Sensitized Solar Cells
Ameri, Mohsen; Raoufi, Meysam; Zamani-Meymian, M. -R.; Samavat, Feridoun; Fathollahi, M. -R.; Mohajerani, Ezeddin (2018-03-01)
High surface area and enhanced light scattering of ZnO nanosheet aggregates have made them a promising active layer candidate material for fabrication of nanostructure dye-sensitized solar cells. Here, we propose a facile preparation method of such ZnO nanosheet structures, and in order to verify their applicability as photoanode material for dye-sensitized solar cells, we employ morphological, optical, structural and electrical measurements. The results reveal the high surface area available for dye molecu...
Flexible chiral metamaterials with dynamically optical activity and high negative refractive index
Dincer, Furkan; KARAASLAN, MUHARREM; ÜNAL, EMİN; Akgol, Oguzhan; Sabah, Cumali (2015-07-10)
We demonstrate numerically and experimentally chiral metamaterials (MTMs) based on gammadion-bilayer cross-wires that uniaxially create giant optical activity and tunable circular dichroism as a result of the dynamic design. In addition, the suggested structure gives high negative refractive index due to the large chirality in order to obtain an efficient polarization converter. We also present a numerical analysis in order to show the additional features of the proposed chiral MTM in detail. Therefore, a M...
Citation Formats
M. Mahmoudian, U. Celikkol, M. Yucel, and H. A. Öktem, “Vacuum infiltration based agrobacterium mediated gene transfer to lentil (Lens culinaris M.) tissues,” BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, pp. 24–29, 2002, Accessed: 00, 2020. [Online]. Available: