A multiplex primer design algorithm for target amplification of continuous genomic regions

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10.1186-s12859-017-1716-7.pdf

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2017-06-19

Author

OZTURK, Ahmet Rasit
Can, Tolga

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Background: Targeted Next Generation Sequencing (NGS) assays are cost-efficient and reliable alternatives to Sanger sequencing. For sequencing of very large set of genes, the target enrichment approach is suitable. However, for smaller genomic regions, the target amplification method is more efficient than both the target enrichment method and Sanger sequencing. The major difficulty of the target amplification method is the preparation of amplicons, regarding required time, equipment, and labor. Multiplex PCR (MPCR) is a good solution for the mentioned problems.

Subject Keywords

Next Generation sequencing, Target amplification, Multiplex PCR, Primer design

URI

https://hdl.handle.net/11511/35742

Journal

BMC BIOINFORMATICS

DOI

https://doi.org/10.1186/s12859-017-1716-7

Collections

Department of Computer Engineering, Article

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Citation Formats

A. R. OZTURK and T. Can, “A multiplex primer design algorithm for target amplification of continuous genomic regions,” BMC BIOINFORMATICS, pp. 0–0, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35742.