Biochemical and Molecular Characterization of Strontium-resistant Environmental Isolates of Pseudomonas fluorescens and Sphingomonas paucimobilis

Ozer, Gamze
Ergene, Aysun
İçgen, Bülent
Strontium resistance was investigated in strains isolated from the river Kzlrmak which has been affected by industrial and agricultural heavy metal pollution from the surrounding facilities and domestic effluents from the city Krkkale, Turkey. Strontium-resistant isolates with a minimal inhibitory concentration of 2000mg L-1 were isolated and identified as Pseudomonas fluorescens and Sphingomonas paucimobilis. Both isolates were shown to be resistant to other heavy metals like aluminum, lithium, barium, nickel and resistance to the antibiotics like aztreonam and pefloxacin. Plasmid DNA analysis, transformation and curing results revealed that the strontium resistance ability of both isolates was chromosome-encoded. Outer membrane and total protein isolation results descriptively showed that not only membrane porins but also other proteins were effective in the strontium resistance in Pseudomonas fluorescens and Sphingomonas paucimobilis.


Environmental geological investigations at the Van open dump site, southeastern Turkey
Akgün, Haluk (Springer Science and Business Media LLC, 2000-04-01)
This study evaluates the geological, hydrogeological and hydrological conditions at the I waste dump site of the Van municipality in eastern Anatolia, Turkey. A geological map and a three-dimensional view of the project site have prepared. All lithological units exposed at and near the dump site have been determined and their boundary relationships identified. Drainage basin characteristics of the dump site along with the water bearing properties of different lithological units have been assessed. For the r...
Experimental Study on Maize Cob Trickling Filter-Based Wastewater Treatment System: Design, Development, and Performance Evaluation
Ali, Imran; Khan, Zahid M.; Sultan, Muhammad; Mahmood, Muhammad H.; Farid, Hafiz U.; Ali, Mohsin; Nasir, Abdul (HARD Publishing Company, 2016-01-01)
In developing countries, good-quality water is contaminated due to the disposal of untreated municipal and industrial wastewater (WW) into natural water reservoirs. Most of the wastewater is not treated properly according to international standards, and usually is disposed of and/or utilized for irrigation without appropriate treatment. The main hurdles in providing wastewater treatment (WWT) in developing countries include high costs, and the poor design, installation, and operation of conventional WWT sys...
Quality assessment of armourstone for a rubble mound breakwater (Sinop, Turkey)
Topal, Tamer (Springer Science and Business Media LLC, 2004-10-01)
Helaldi is a fishing town in the central Black Sea region where construction of a rubble mound breakwater is being planned. The construction of the breakwater requires good quality durable armourstone. Several laboratory tests are performed for the quality assessment of the two potential armourstone, namely, limestone and sandstone. The quality evaluation of the stones is done on the basis of CIRIA/CUR criteria, saturation coefficient, wet-to-dry strength ratio, and the rock durability index. The quality ev...
Biosorption of Strontium from Aqueous Solutions by Micrococcus luteus Sr02
İçgen, Bülent (Informa UK Limited, 2018-01-01)
Although strontium (Sr2+) is found in soils, sediments and surface waters, there is limited evidence about its biosorption. In this study, a surface water strain being highly tolerant to Sr2+ was isolated and identified as Micrococcus luteus Sr02 by using 16S rRNA sequencing. Biosorption behavior and mechanisms of Sr2+ by M. luteus Sr02 were investigated through batch experiments, zeta potential measurements, Fourier transform infrared spectroscopy and scanning electron microscopy. The results showed that M...
Contamination of the cement raw material in a quarry site by seawater intrusion, Darica-Turkey
Çamur, Mehmet Zeki (Springer Science and Business Media LLC, 2008-02-01)
The open pit mining nearby shoreline is planned to be extended into below sea level in order to use additional reserves of the cement raw material (marl). The raw material is currently contaminated by seawater intrusion below a depth of 20 m up to the distance of 90 m from shoreline. Seawater intrusion related contamination of the material used for the cement production was investigated by means of diffusion process for the future two below sea level mining scenarios covering 43 years of period. According t...
Citation Formats
G. Ozer, A. Ergene, and B. İçgen, “Biochemical and Molecular Characterization of Strontium-resistant Environmental Isolates of Pseudomonas fluorescens and Sphingomonas paucimobilis,” GEOMICROBIOLOGY JOURNAL, pp. 381–390, 2013, Accessed: 00, 2020. [Online]. Available: