Fabrication of a Novel Polymeric Scaffold for Amperometric Laccase Biosensor

Soylemez, Saniye
Bekmezci, Aybüke
Göker, Seza
Toppare, Levent Kamil
Present work displays the preparation of an electrochemical biosensor using a conjugated polymer and laccase enzyme for catechol quantification in samples. The biosensing system is based on an enzyme immobilization on polymer modified graphite transducer surface. For that purpose, a random conjugated polymer, thienothiophene-benzoxadiazole-alt-benzodithiophene (BOTT), was coated onto a graphite electrode surface via drop casting method followed by immobilization of a biomolecule (laccase) for sensing experiments. Herein, for the first time, we proposed a BOTT polymer as an inexpensive and effective way to fabricate highly sensitive and fast response biosensors. The proposed sensing system possessed superior properties with 0.38 mu M limit of detection and 110.81 mu AmM-1 sensitivity. Furthermore, cyclic voltammetry and scanning electron microscopy techniques were used to examine the surface modifications. The proposed system could be useful for many future studies for catechol quantification in environmental samples.(c) 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019


A new soluble conducting polymer and its electrochromic devices
YİLDİZ, UMİT HAKAN; SAHİN, ELİF; Akhmedov, IM; Tanyeli, Cihangir; Toppare, Levent Kamil (Wiley, 2006-04-01)
A new polythiophene derivative was synthesized by both chemical and electrochemical oxidative polymerization of 1-(1-phenylethyl)-2,5-di(2-thienyl)-1H-pyrrole (PETPy). Of which the chemical method produces a polymer that is completely soluble in organic solvents. The structures of both the monomer and the soluble polymer were elucidated by nuclear magnetic resonance (H-1 and C-13 NMR) and Fourier transform infrared (FTIR) spectroscopy. The average molecular weight has been determined by gel permeation chrom...
Synthetic Design of Polyester Electrolytes Guided by Hydrophobicity Calculations
Yıldırım, Erol; Peretic, Matthew J.; Pasquinelli, Melissa A.; Mathers, Robert T. (American Chemical Society (ACS), 2016-10-25)
Partition coefficients (LogP) help to quantify hydrophobicity, which can be used to guide the design of polymer electrolytes with properties. Thus, this study combined synthetic experiments and modeling to produce polyester electrolytes that solubilize lithium salts. These polyester electrolytes were derived from natural sources and polymerized with different ratios of polyols (diglycerol, glycerol, and diethylene glycol) and citric acid in the presence of lithium salts (LiTf and LiTFSI). The Fisher esterif...
Synthesis, characterization and electrochromic properties of a near infrared active conducting polymer of 1,4-di(selenophen-2-yl)-benzene
Aydemir, Kadir; Tarkuc, Simge; Durmus, Asuman; Günbaş, Emrullah Görkem; Toppare, Levent Kamil (Elsevier BV, 2008-04-15)
A novel selenophene-based monomer, 1,4-di(selenophen-2-yl)-benzene (DSB), was synthesized via Stille coupling reaction of 1,4-dibromobenzene and tributyl(2-selenophenyl)stannane. Conducting polymer (PDSB) was prepared electrochemically in the presence of tetrabutylammonium hexafluorophosphate (TBAPF(6)) as the supporting electrolyte in dichloromethane (DCM). The resulting conducting polymer was characterized by Cyclic Voltammetry, Fourier Transform Infrared and Ultraviolet-visible spectroscopy. Spectroelect...
Fused structures in the polymer backbone to investigate the photovoltaic and electrochromic properties of donoracceptor-type conjugated polymers
CEVHER, ŞEVKİ CAN; UNLU, Naime Akbasoglu; OZELCAGLAYAN, Ali Can; APAYDIN, Dogukan Hazar; Udum, Yasemin Arslan; Toppare, Levent Kamil; Çırpan, Ali (Wiley, 2013-05-01)
In this study, two new benzotriazole (BTz) and dithienothiophene (DTT) containing conjugated polymers were synthesized. After successful characterizations of the monomers by proton-nuclear magnetic resonance (1H NMR) and carbon-NMR (13C NMR) techniques, poly(4-(dithieno[3, 2-b:2,3-d]thiophen-2-yl)-2-(2-octyldodecyl)-2H-benzo[d][1,2,3] triazole) P1 and poly(4-(5-(dithieno[3,2-b:2,3-d]thiophen-2-yl)thiophen-2-yl)-2-(2-octyldodecyl)-7-(thiophen-2-yl)-2H-benzo[d][1,2,3]triazole) P2 were synthesized via a typica...
Immobilization of Invertase in a Novel Proton Conducting Poly(vinylphosphonic acid) - poly(1-vinylimidazole) Network
Isikli, Suheda; Tuncagil, Sevinc; Bozkurt, Ayhan; Toppare, Levent Kamil (Informa UK Limited, 2010-01-01)
A novel proton conducting polymer blend was prepared by mixing poly(vinylphosphonic acid) (PVPA) with poly(1-vinylimidazole) (PVI) at various stoichiometric ratios via changing molar ratio of monomer repeating unit to achieve the highest protonation. The polymer network having the most suitable stoichiometric ratio for substantial proton conductivity was prepared and characterized by FT-IR spectroscopy and proton conductivity measurements. The network was used for immobilization of invertase and some import...
Citation Formats
S. Soylemez, A. Bekmezci, S. Göker, and L. K. Toppare, “Fabrication of a Novel Polymeric Scaffold for Amperometric Laccase Biosensor,” JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, pp. 2333–2339, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35822.