Fabrication of a Novel Polymeric Scaffold for Amperometric Laccase Biosensor

Date

2019-11-14

Author

Soylemez, Saniye
Bekmezci, Aybüke
Göker, Seza
Toppare, Levent Kamil

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Present work displays the preparation of an electrochemical biosensor using a conjugated polymer and laccase enzyme for catechol quantification in samples. The biosensing system is based on an enzyme immobilization on polymer modified graphite transducer surface. For that purpose, a random conjugated polymer, thienothiophene-benzoxadiazole-alt-benzodithiophene (BOTT), was coated onto a graphite electrode surface via drop casting method followed by immobilization of a biomolecule (laccase) for sensing experiments. Herein, for the first time, we proposed a BOTT polymer as an inexpensive and effective way to fabricate highly sensitive and fast response biosensors. The proposed sensing system possessed superior properties with 0.38 mu M limit of detection and 110.81 mu AmM-1 sensitivity. Furthermore, cyclic voltammetry and scanning electron microscopy techniques were used to examine the surface modifications. The proposed system could be useful for many future studies for catechol quantification in environmental samples.(c) 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019

Subject Keywords

Organic Chemistry, Materials Chemistry, Polymers and Plastics

URI

https://hdl.handle.net/11511/35822

Journal

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY

DOI

https://doi.org/10.1002/pola.29537

Collections

Department of Chemistry, Article

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Citation Formats

S. Soylemez, A. Bekmezci, S. Göker, and L. K. Toppare, “Fabrication of a Novel Polymeric Scaffold for Amperometric Laccase Biosensor,” JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, pp. 2333–2339, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35822.