Abiotic stress tolerance and growth responses of transgenic potato (Solanum tuberosum L. cv. Kennebec) plants expressing rice Osmyb4 gene

Date

2012-09-23

Author

AYDIN, GÜLSÜM
Yucel, Meral
Öktem, Hüseyin Avni

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MYB transcription factors are involved in diverse biochemical and physiological processes such as regulation of secondary metabolism, meristem formation, cell morphogenesis and floral and seed development. They are also involved in certain defence and stress responses and in hormone signalling. In the present study, we developed transgenic potato (Solanum tuberosum L. cv. Kennebec) expressing Oryza sativa myb4 gene, encoding MYB4 transcription factor, driven by either CaMV35S constitutive promoter or cold inducible COR15a promoter. The transformation frequencies were 37.6% (pCORmyb4) and 27.5% (pCaMVmyb4). A total of four pCOR lines and three pCaMV lines were selected randomly for bioassays. The transgenic plants were not growth retarded and there was no significant difference (p < 0.05) in their tuber yield compared to non-transgenic wild type plants. Although chlorophyll a, chlorophyll b and anthocyanin contents of transgenic lines were similar to wild type, one of the pCaMV lines had a significantly higher carotenoid content. The total sugar contents of two lines were also significantly higher. Transgenic plants were also evaluated for abiotic stress tolerances. There was no significant difference in boron and freezing tolerances of wild type and transgenic lines. However a pCOR line and a pCaMV line were more salt tolerant than wild type with respect to growth parameters. These results demonstrated that heterologous expression of myb4 in potato affects carotenoid and total sugar content and also enhances salt stress tolerance. The results also showed that effect of myb4 expression might be different in each transgenic line under the conditions tested.

Subject Keywords

Carotenoid, Potato, Agrobacterium-mediated gene transfer, Abiotic stress, myb4

URI

https://hdl.handle.net/11511/35892

Journal

NEW BIOTECHNOLOGY

DOI

https://doi.org/10.1016/j.nbt.2012.08.068

Collections

Department of Biology, Article

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Citation Formats

G. AYDIN, M. Yucel, and H. A. Öktem, “Abiotic stress tolerance and growth responses of transgenic potato (Solanum tuberosum L. cv. Kennebec) plants expressing rice Osmyb4 gene,” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35892.