Expression system for synthesis and purification of recombinant human growth hormone in Pichia pastoris and structural analysis by MALDI-ToF mass spectrometry

Date

2008-01-01

Author

Çalık, Pınar
Celik, Eda
Halloran, S. Mitchell
Calik, Guezide
Ozdamar, Tuncer H.

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An expression system in Pichia pastoris for the production and purification of recombinant human growth hormone (rHGH) was designed and implemented. hGH cDNA sequence was cloned into pPICZ alpha A vector under the control of AOX1 promoter, which included a polyhistidine-tag on the amino terminal end to enable affinity purification and a target site for Factor Xa protease such that protease cleavage in vitro would produce rhGH without any non-native Nand C-termini. Analyses of the affinity-purified rhGH product by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) showed a spectral peak at m/z 23699. Purified product digested with Factor Xa protease had a molecular mass of 22132 kDa. The molecular mass difference before and after Factor Xa protease digestion expectedly corresponds to the 12 amino acids in the rhGH amino terminus, which includes the EcoRI digestion site (Glu-Phe), the 6xHis tag for affinity purification, and the Factor Xa protease recognition sequence (Ile-Glu-Gly-Arg), a result that also indicates that the signal peptide was properly processed by P. pastoris. N-Terminal sequence analysis of the Factor Xa protease trimmed recombinant product confirmed the mature hGH sequence. Thus, the system designed functioned with its intended purpose effectively in expression, cleavage, and purification of the recombinant product.

Subject Keywords

Biotechnology

URI

https://hdl.handle.net/11511/35930

Journal

BIOTECHNOLOGY PROGRESS

DOI

https://doi.org/10.1021/bp070294t

Collections

Department of Chemical Engineering, Article

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Citation Formats

P. Çalık, E. Celik, S. M. Halloran, G. Calik, and T. H. Ozdamar, “Expression system for synthesis and purification of recombinant human growth hormone in Pichia pastoris and structural analysis by MALDI-ToF mass spectrometry,” BIOTECHNOLOGY PROGRESS, pp. 221–226, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/35930.