Correlation between hippocampal levels of neural, epithelial and inducible NOS and spatial learning skills in rats

Gokcek-Sarac, Cigdem
Karakurt, Serdar
Adalı, Orhan
Jakubowska-Dogru, Ewa
In the present study, to better understand the role of different nitric oxide synthase (NOS) isoforms in hippocampus-dependent forms of learning, we examined the expression of neural, endothelial, and inducible NOS in the hippocampus of young-adult rats classified as "poor" and "good" learners on the basis of their performance in the partially baited 12-arm radial maze. Taking into consideration strain-dependent differences in learning skills and NOS expression, experiments were performed on two different lines of laboratory rats: the inbred Wistar (W) and the outcrossed Wistar/Spraque-Dawley(W/S) line. The hippocampal levels of NOS proteins were assessed by Western Blotting. In the present study, genetically more homogenous W rats showed a slower rate of learning compared to the genetically less homogenous outcrossed W/S rats. The deficient performance in the W rat group compared to outcrossed W/S rats, and in "poor" learners of both groups compared to "good" learners was due to a higher percentage of reference memory errors. The overall NOS levels were significantly higher in W group compared to outcrossed W/S rats. In both rat lines, the rate of learning positively correlated with hippocampal levels of nNOS and negatively correlated with iNOS levels. Hippocampal eNOS levels correlated negatively with animals' performance but only in the W rats. These results suggest that all 3 NOS isoforms are implemented but play different roles in neural signaling.


Relationship between the hippocampal expression of selected cytochrome P450 isoforms and the animal performance in the hippocampus-dependent learning task
Gjota-Ergin, Sena; Gokcek-Sarac, Cigdem; Adalı, Orhan; Jakubowska-Dogru, Ewa (2018-04-23)
Despite very extensive studies on the molecular mechanisms of memory formation, relatively little is known about the molecular correlates of individual variation in the learning skills within a random population of young normal subjects. The role of cytochrome P450 (CYP) enzymes in the brain also remains poorly understood. On the other hand, these enzymes are known to be related to the metabolism of substances important for neural functions including steroids, fatty acids, and retinoic acid. In the present ...
Comparison of spatial learning in the partially baited radial-arm maze task between commonly used rat strains: Wistar, Spargue-Dawley, Long-Evans, and outcrossed Wistar/Sprague-Dawley
Gokcek-Sarac, Cigdem; Wesierska, Malgorzata; Jakubowska-Dogru, Ewa (2015-03-01)
Strain-related differences in animals' cognitive ability affect the outcomes of experiments and may be responsible for discrepant results obtained by different research groups. Therefore, behavioral phenotyping of laboratory animals belonging to different strains is important. The aim of the present study was to compare the variation in allothetic visuospatial learning in most commonly used laboratory rat strains: inbred Wistar (W) and Sprague-Dawley (SD), outcrossed Wistar/Sprague-Dawley (W/SD), and outbre...
Study on the molecular basis of individual variation in spatial memory in rats
Gökçek Saraç, Çiğdem; Jakubowska Doğru, Ewa; Adalı, Orhan; Department of Biology (2012)
Despite very extensive studies related to molecular processes underlying memory formation, still little known about the potential differences in the brain biochemistry between “good” and “poor” learners belonging to a random population of young animals. In the present study, an attempt was taken to correlate the individual variation in short- and long-term spatial memory in three different lines of young, healthy rats: inbred Wistar (W), outcrossed Wistar/Spraque Dawley (W/S) and pigmented Long-Evans rats, ...
Analysis of immunoreactivity of NOS isoforms (nNOS, eNOS, iNOS) in hippocampus of young rats classified as good and poor learners
Keçecioğlu, Ekin; Jakubowska Doğru, Ewa; Department of Biology (2012)
Despite very extensive studies on molecular mechanisms of learning and memory formation it is little known about individual variation in the learning skills within a random animal population and about the differences in the brain biochemistry behind this variation. In the present study, we have focused on the expression and distribution of nitric oxide synthase (NOS), one of the molecules implemented in activity-dependent neuroplasticity, in the rat hippocampus, the structure critical for episodic memory in...
Individual variation in the spatial reference and working memory assessed under allothetic and idiothetic orientation cues in rat
Jakubowska-Dogru, E; Gumusbas, U; Kara, F (2003-01-01)
The present study was designed to examine which kind of memory: reference or working, better correlates with individual variation in rats' spatial learning abilities. To answer this question two groups of rats were trained to an arbitrary criterion in a partially baited 12-arm radial maze under two different experimental conditions: with or without allothetic cues. After 10 days break, rats were examined under the same conditions for memory retention. Within- and between-group variation in the length of tra...
Citation Formats
C. Gokcek-Sarac, S. Karakurt, O. Adalı, and E. Jakubowska-Dogru, “Correlation between hippocampal levels of neural, epithelial and inducible NOS and spatial learning skills in rats,” BEHAVIOURAL BRAIN RESEARCH, pp. 326–333, 2012, Accessed: 00, 2020. [Online]. Available: