Detoxification of Groundnut Cake Naturally Contaminated with Aflatoxin B-1 Using Rhodococcus erythropolis in Shake Flask Bioreactors

Date

2017-04-01

Author

Dogan, Onay Burak
ÖNAL ULUSOY, BARAN
Bozoglu, Faruk
Sagdicoglu-Celep, Adviye Gulcin
Çekmecelioğlu, Deniz

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

192
views

0
downloads

Degradation of aflatoxin B-1 in groundnut cake, a residue after oil extraction, by Rhodococcus erythropolis was optimized using response surface methodology. The bacterium was first grown in synthetic medium to determine optimal growth conditions using the combination of the Plackett-Burman and Box-Behnken methods and consequently aflatoxin B-1 in groundnut cake slurry was degraded under varying culture conditions. Optimal growth conditions were found as 22.5 A degrees C of temperature, pH 7, 100 mL of culture volume in 500 mL flasks, 1 % (v/v) of inoculum size, 135 rpm of agitation speed, 5 g/L of glucose and 5 g/L of peptone concentration according to analysis of the Box-Behnken design. Optimal detoxification conditions were found as 27.4 % (w/v) of solid concentration, 4.88 % (v/v) of inoculum size and 24 h of incubation time by Box-Behnken response surface optimization. Maximum detoxification level was predicted as 92.2 % by the constructed model while the experimental counterpart was 87.3 %. The suggested culture conditions have the potential to decrease aflatoxin B-1 from 200 mu g/kg to below 20 mu g/kg, the regulatory limit, in feed materials. Further studies are needed to test the obtained results at a larger scale and to finalize safety assessment of the final product by animal testing.

Subject Keywords

Renewable Energy, Sustainability and the Environment, Environmental Engineering, Waste Management and Disposal

URI

https://hdl.handle.net/11511/37848

Journal

WASTE AND BIOMASS VALORIZATION

DOI

https://doi.org/10.1007/s12649-016-9598-x

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Effects of High Hydrostatic Pressure (HHP) Processing and Temperature on Physicochemical Characterization of Insect Oils Extracted from Acheta domesticus (House Cricket) and Tenebrio molitor (Yellow Mealworm) Ugur, Ahmet Erdem; Bolat, Berkay; Öztop, Halil Mecit; Alpas, Hami (Springer Science and Business Media LLC, 2020-11-01) Oil portion of Tenebrio molitor (yellow mealworm) and Acheta domesticus (house cricket) were examined and it was investigated how the physicochemical properties of the oils changed with High Hydrostatic Pressure Assisted Extraction (HHP-E) and conventional solvent extraction (CE) with hexane. The effect of HHP-E at 500 MPa and 30 and 40 degrees C for 15 min on the properties of oils was compared with the CE. Following the extraction of oil, fatty acid composition, peroxide value, crystallization and melting...
The inhibitory effects and removal of dieldrin continuous upflow anaerobic sludge blanket reactors Bayramoğlu, Tuba Hande; DEMİRER, GÖKSEL NİYAZİ (Elsevier BV, 2003-09-01) The inhibitory effects and removal efficiency of dieldrin (DLD) in anaerobic reactors were investigated. Anaerobic toxicity assay (ATA) experiments conducted in batch reactors revealed that 30 mg/l DLD had inhibitory effects on the unacclimated mixed anaerobic cultures. Continuous reactor experiments performed in a lab-scale two-stage upflow anaerobic sludge blanket (UASB) reactor system which was fed with ethanol as the sole carbon source, indicated that anaerobic granular cultures could be successfully ac...
Production of Cellulase and Xylanase Enzymes Using Distillers Dried Grains with Solubles (DDGS) by Trichoderma reeseiat Shake-Flask Scale and the Validation in the Benchtop Scale Bioreactor Çekmecelioğlu, Deniz (Springer Science and Business Media LLC, 2020-01-01) Most of the corn-ethanol plants use dry-mill process and generate large volume of distillers dried grains with solubles (DDGS) as the co-product. The DDGS is a promising feedstock with rich content of cellulose and xylan. The goal of this study was to optimize cellulase and xylanase production byTrichoderma reesei(NRRL 6156) using DDGS as the carbon source at shake-flask and validate the results at benchtop bioreactors. The fermentation medium containing varied amounts of dilute-acid pretreated DDGS, yeast ...
Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (Elsevier BV, 2019-06-01) Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreat...
Removal of Ni(II) Ions From Aqueous Solutions Using Activated-Carbon Prepared From Olive Stone by ZnCl2 Activation UĞURLU, MEHMET; KULA, İBRAHİM; KARAOĞLU, MUHAMMET HAMDİ; Arslan, Yasin (Wiley, 2009-12-01) The aim of this study is to remove Ni (II) ions from aqueous solutions by adsorption. Activated-carbon prepared from olive stone (ACOS) was used as adsorbent. Different particle size and concentration of ZnCl2 were studied to optimize adsorbent surface area. Initial concentration, temperature, time, and pH were selected as parameters. According to the experiments results, the equilibrium time, optimum pH, and adsorbent dosage were found 60 min, pH > 6, and 1.0 g/50 mL, respectively. In addition, raw olive s...

Citation Formats

O. B. Dogan, B. ÖNAL ULUSOY, F. Bozoglu, A. G. Sagdicoglu-Celep, and D. Çekmecelioğlu, “Detoxification of Groundnut Cake Naturally Contaminated with Aflatoxin B-1 Using Rhodococcus erythropolis in Shake Flask Bioreactors,” WASTE AND BIOMASS VALORIZATION, pp. 721–731, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37848.