An LCMSMS method to analyse phenolic profile in the liquid extract, with woodland strawberry (Fragaria vesca l.) application

Şeker, Tamay
Yucel, Meral
The most of the bioactive compounds are found in trace amounts so, an efficient extraction procedure from a given tissue is a big problem. An efficient quantitative detection coupled to the extraction procedure is necessary in order to cover the whole phenomena. The increasing dissolution volume of the lyophilized sample may result in dilution of the total content of the phenolics. In choosing the analytical measurement method, the lower sample-enjection volume, the seperate detection of the phenolics having same UV absorbance, short run time and the sensitivity are the important parameters to prefer LCMSMS compared to the classical HPLC. We designed an analytical measurement method via liquid chromatography tandem mass spectrometry with both positive and negative electrospary ionization. MS grade methanol (B) and 5 mM ammonium formate (negative ionisation) with 0.5% formic acid (positive ionisation) (A) were used as mobile phase in gradient grade profile. 5 μL enjection volume was enough for the repetetive results. At least 25 different phenolic compounds could be quantified through 13 min run time in 0.01–25 ppm range. The method was applied for the woodland strawberry samples extracted in two different methods together with their upper ground parts. Since there was no a study informing the phenolic content of the woodland strawberry via LCMSMS, it was seen that the optimized method is a better alternative compared to HPLC. The method can be used for any liquid sample for the phenolic profile analyses.


A simple synthesis of 1-aminophosphonic acids from 1-hydroxyiminophosphonates with NaBH4 in the presence of transition metal compounds
Demir, AS; Tanyeli, Cihangir; Sesenoglu, O; Demic, S; Evin, OO (1996-01-15)
A new procedure has been developed for the synthesis of 1-aminophosphonic acids. Diethyl phosphonates are converted to hydroxyiminophosphonates when treated with hydroxylamine hydrochloride. Reduction of hydroxyiminophosphonates with NaBH4 in MeOH in the presence of MoO3 or NiCl2 and hydrolysis of 1- aminophosphonates gave 1-aminophosphonic acids in good yield.
A DFT study on nitrotriazines
Türker, Burhan Lemi; Atalar, Taner; Gumus, Selcuk; Camur, Yakup (2009-08-15)
In this study, all possible mono-, di- and tri-nitro-substituted triazine compounds have been considered as potential candidates for high-energy density materials (HEDMs) by using quantum chemical treatment. Geometric and electronic structures, thermodynamic properties and detonation performances of these nitro-substituted triazines have been systematically studied using density functional theory (DFT, B3LYP) at the level of 6-31G(d,p), 6-31+G(d,p), 6-311G(d,p), 6-311+G(d,p) and cc-pVDZ basis sets. Moreover...
A theoretical study on coenzyme Q(10) and its reduced form. AM1 treatment
Türker, Burhan Lemi (2000-01-01)
Coenzyme Q(10) (CoQ(10)) and its reduced form, ubiquinol (CoQ(10)H(2)) were subjected to semiempirical quantum chemical treatment at the level of AM1-RHF type calculations. Both of the structures are stable having negative total and binding energies and exothermic heats of formation. The frontier molecular orbital characteristics, electrostatic contour diagrams and charge distributions of these structures were presented.
Novel Mesoporous Nanocomposite WOx-Silicate Acidic Catalysts: Ethylene and Diethylether from Ethanol
Varisli, Dilek; Doğu, Timur; DOĞU, GÜLŞEN (2009-11-04)
Solid acid catalysts are extensively used in a number of industrially important catalytic reactions. Here, water-insoluble novel mesoporous solid acid catalysts, with a unique structure composed of WOx nanorods and W nanoballs within an amorphous silicate lattice, were synthesized following a one-pot hydrothermal route. using silicotungstic acid (STA) as the active component. Surface area of these catalysts ire more than 2 orders of magnitude higher than that of pure STA, and they showed very high activity ...
A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
Soldatkina, O. V.; Soldatkin, O. O.; Kasap, B. Ozansoy; Kucherenko, D. Yu.; Kucherenko, I. S.; Akata Kurç, Burcu; Dzyadevych, S. V. (2017-04-07)
In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed...
Citation Formats
T. Şeker, A. TÜRKER, and M. Yucel, “An LCMSMS method to analyse phenolic profile in the liquid extract, with woodland strawberry (Fragaria vesca l.) application,” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: