An LCMSMS method to analyse phenolic profile in the liquid extract, with woodland strawberry (Fragaria vesca l.) application

Date

2012-09-23

Author

Şeker, Tamay
TÜRKER, ARZU
Yucel, Meral

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The most of the bioactive compounds are found in trace amounts so, an efficient extraction procedure from a given tissue is a big problem. An efficient quantitative detection coupled to the extraction procedure is necessary in order to cover the whole phenomena. The increasing dissolution volume of the lyophilized sample may result in dilution of the total content of the phenolics. In choosing the analytical measurement method, the lower sample-enjection volume, the seperate detection of the phenolics having same UV absorbance, short run time and the sensitivity are the important parameters to prefer LCMSMS compared to the classical HPLC. We designed an analytical measurement method via liquid chromatography tandem mass spectrometry with both positive and negative electrospary ionization. MS grade methanol (B) and 5 mM ammonium formate (negative ionisation) with 0.5% formic acid (positive ionisation) (A) were used as mobile phase in gradient grade profile. 5 μL enjection volume was enough for the repetetive results. At least 25 different phenolic compounds could be quantified through 13 min run time in 0.01–25 ppm range. The method was applied for the woodland strawberry samples extracted in two different methods together with their upper ground parts. Since there was no a study informing the phenolic content of the woodland strawberry via LCMSMS, it was seen that the optimized method is a better alternative compared to HPLC. The method can be used for any liquid sample for the phenolic profile analyses.

Subject Keywords

Phenolics, LCMSMS, Woodland strawberry, HPLC

URI

https://hdl.handle.net/11511/39061

Journal

NEW BIOTECHNOLOGY

DOI

https://doi.org/10.1016/j.nbt.2012.08.469

Collections

Test and Measurement Center In advanced Technologies (MERKEZ LABORATUVARI), Article

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Citation Formats

T. Şeker, A. TÜRKER, and M. Yucel, “An LCMSMS method to analyse phenolic profile in the liquid extract, with woodland strawberry (Fragaria vesca l.) application,” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39061.