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A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
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10.1186-s11671-017-2026-8.pdf
Date
2017-04-07
Author
Soldatkina, O. V.
Soldatkin, O. O.
Kasap, B. Ozansoy
Kucherenko, D. Yu.
Kucherenko, I. S.
Akata Kurç, Burcu
Dzyadevych, S. V.
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In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 mu M, and the limit of glutamate detection was 1 mu M. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.
Subject Keywords
Silicalite
,
Enzyme
,
Biosensor
,
Glutamate oxidase
,
Amperometry
URI
https://hdl.handle.net/11511/32432
Journal
NANOSCALE RESEARCH LETTERS
DOI
https://doi.org/10.1186/s11671-017-2026-8
Collections
Graduate School of Natural and Applied Sciences, Article
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O. V. Soldatkina et al., “A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite,”
NANOSCALE RESEARCH LETTERS
, pp. 0–0, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/32432.