Effect of suppressive DNA on CpG-induced immune activation.

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2002-11-15

Author

Yamada, H
Gursel, I
Takeshita, F
Conover, J
Ishii, KJ
Gürsel, Mayda
Takeshita, S
Klinman, DM

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Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs stimulate a strong innate immune response. This stimulation can be abrogated by either removing the CpG DNA or adding inhibitory/suppressive motifs. Suppression is dominant over stimulation and is specific for CpG-induced immune responses (having no effect on LPS- or Con A-induced activation). Individual cells noncompetitively internalize both stimulatory and suppressive ODN. Studies using ODN composed of both stimulatory and suppressive motifs indicate that sequence recognition proceeds in a 5'-->3' direction, and that a 5' motif can block recognition of immediately 3' sequences. These findings contribute to our understanding of the immunomodulatory activity of DNA-based products and the rules that govern immune recognition of stimulatory and suppressive motifs.

Subject Keywords

Immunology

URI

https://hdl.handle.net/11511/39212

Journal

Journal of immunology (Baltimore, Md. : 1950)

DOI

https://doi.org/10.4049/jimmunol.169.10.5590

Collections

Department of Biology, Article

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Citation Formats

H. Yamada et al., “Effect of suppressive DNA on CpG-induced immune activation.,” Journal of immunology (Baltimore, Md. : 1950), pp. 5590–4, 2002, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/39212.