Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate

Date

2015-06-01

Author

Moral, Cigdem Kivilcimdan
Doğan, Özdemir
Sanin, Faika Dilek

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Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers. It is produced by algae and some species of Azotobacter and Pseudomonas. This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCCA (R) 9046 in a fermenter. Results showed that alginate production increased with increasing DOT from 1 to 5 %. The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT. At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration. On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).

Subject Keywords

Biotechnology, Applied Microbiology and Biotechnology, Biochemistry, Bioengineering, Molecular Biology, General Medicine

URI

https://hdl.handle.net/11511/40035

Journal

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1007/s12010-015-1617-z

Collections

Department of Chemistry, Article

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Citation Formats

C. K. Moral, Ö. Doğan, and F. D. Sanin, “Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate,” APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, pp. 875–891, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40035.