Draw your assay: Fabrication of low-cost paper-based diagnostic and multi-well test zones by drawing on a paper

Date

2015-06-22

Author

OYOLA-REYNOSO, Stephanie
HEIM, Andrew P.
HALBERTSMA-BLACK, Julian
ZHAO, C.
TEVIS, Ian D.
Çınar, Simge
Cademartiri, Rebecca
LIU, Xinyu
Bloch, Jean-Francis
Thuo, Martin M.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

154
views

0
downloads

Interest in low-cost diagnostic devices has recently gained attention, in part due to the rising cost of healthcare and the need to serve populations in resource-limited settings. A major challenge in the development of such devices is the need for hydrophobic barriers to contain polar bio-fluid analytes. Key approaches in lowering the cost in diagnostics have centered on (i) development of low-cost fabrication techniques/processes, (ii) use of affordable materials, or, (iii) minimizing the need for high-tech tools. This communication describes a simple, low-cost, adaptable, and portable method for patterning paper and subsequent use of the patterned paper in diagnostic tests. Our approach generates hydrophobic regions using a ball-point pen filled with a hydrophobizing molecule suspended in a solvent carrier. An empty ball-point pen was filled with a solution of trichloro perfluoroalkyl silane in hexanes (or hexadecane), and the pen used to draw lines on Whatman (R) chromatography 1 paper. The drawn regions defined the test zones since the trichloro silane reacts with the paper to give a hydrophobic barrier. The formation of the hydrophobic barriers is reaction kinetic and diffusion-limited, ensuring well defined narrow barriers. We performed colorimetric glucose assays and enzyme-linked immuno-sorbent assay (ELISA) using the created test zones. To demonstrate the versatility of this approach, we fabricated multiple devices on a single piece of paper and demonstrated the reproducibility of assays on these devices. The overall cost of devices fabricated by drawing are relatively lower (<US $0.001 per device) than those derived from wax-printing (US$0.05-0.003) or other approaches.

Subject Keywords

Paper-Based Devices, Low-Cost Devices, Point Of Care Diagnostics, Mu-Pads, P-ELISA

URI

https://hdl.handle.net/11511/40162

Journal

Talanta

DOI

https://doi.org/10.1016/j.talanta.2015.06.018

Collections

Department of Metallurgical and Materials Engineering, Article

Suggestions

OpenMETU
Core

Reprint of 'Draw your assay: Fabrication of low-cost paper-based diagnostic and multi-well test zones by drawing on a paper' OYOLA-REYNOSO, Stephanie; HEIM, Andrew P.; HALBERTSMA-BLACK, Julian; ZHAO, C.; TEVIS, Ian D.; Çınar, Simge; Cademartiri, Rebecca; LIU, Xinyu; Bloch, Jean-Francis; Thuo, Martin M. (2015-12-01) Interest in low-cost diagnostic devices has recently gained attention, in part due to the rising cost of healthcare and the need to serve populations in resource-limited settings. A major challenge in the development of such devices is the need for hydrophobic barriers to contain polar bio-fluid analytes. Key approaches in lowering the cost in diagnostics have centered on (i) development of low-cost fabrication techniques/processes, (ii) use of affordable materials, or, (iii) minimizing the need for high-te...
IMPLEMENTATION OF A PATIENT EMPOWERMENT PLATFORM FOR INTEGRATED CARE Sarıgül, Bünyamin; Aydın Son, Yeşim; Laleci Ertürkmen, Gökçe Banu; Department of Bioinformatics (2022-2-11) While the number of chronic disease patients are increasing with the aging population, their complex treatments are a burden in today’s healthcare systems both economically and technically. Integrated care systems are one of the most powerful modern technologies to overcome these difficulties and provide more efficient treatments. Integrated care enables planning the chronic disease treatments in a sustainable and collaborative way for healthcare professionals from various disciplinaries. It also helps care...
Series: The research agenda for general practice/family medicine and primary health care in Europe. Part 2. Results: Primary care management and community orientation Hummers-Pradier, Eva; Beyer, Martin; Chevallier, Patrick; Eilat-Tsanani, Sophia; Lionis, Christos; Peremans, Lieve; Petek, Davorina; Rurik, Imre; Soler, Jean Karl; Stoffers, Henri Ejh; Topsever, Pinar; Ungan, Mehmet; van Royen, Paul (2010-03-01) At the WONCA Europe conference 2009 the recently published 'Research Agenda for General Practice/Family Medicine and Primary Health Care in Europe' was presented. It is a background paper and reference manual, providing advocacy of general practice/family medicine (GP/FM) in Europe. The Research Agenda summarizes the evidence relating to the core competencies and characteristics of the WONCA Europe defi nition of GP/FM, and its implications for general practitioners/family doctors, researchers and policy ma...
Wireless data transmission for medical implants with fully digital non-coherent detection Doğan, Mert; Külah, Haluk; Department of Electrical and Electronics Engineering (2021-9) Number of people awaiting therapeutic devices and implants increase with the increase in population. Cochlear implants are one of these implants which aid patients with sensorineural hearing loss. Conventional cochlear implants consist of two distinct parts inside and outside of the ear. Communication in between these parts is conducted wirelessly. In fully implantable cochlear implants, there is no operational outside block. Hence, in order to interact with all the blocks, wireless data transfer should be ...
Usability evaluation of mobile information and communications technology in health care Akbaşoğlu, Beyza; Baykal, Nazife; Department of Health Informatics (2013) Technology plays an increasingly important role in modern health care. This thesis presents an approach to usability evaluation of mobile information and communications technologies designed for diabetes patients’ use in their daily lives. According to our study conducted on 60 diabetes patients, several important findings were obtained. Fifty nine (98.3%) diabetes patients were highly satisfied with the mobile health technology and expressed that they would use it, and found the measured values reliable. F...

Citation Formats

S. OYOLA-REYNOSO et al., “Draw your assay: Fabrication of low-cost paper-based diagnostic and multi-well test zones by drawing on a paper,” Talanta, pp. 289–293, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40162.