From RNA isolation to microarray analysis: Comparison of methods in FFPE tissues

2016-01-01
Belder, Nevin
Coskun, Oznur
ERDOĞAN, BEYZA DOĞANAY
İlk Dağ, Özlem
SAVAŞ, BERNA
ENSARİ, ARZU
ÖZDAĞ, Hilal
Background: Genome-wide gene expression profiling analysis of FFPE tissue samples is indispensable for cancer research and provides the opportunity to evaluate links between molecular and clinical information, however, working with FFPE samples is challenging due to extensive cross-linking, fragmentation and limited quantities of nucleic acid. Thus, processing of FFPE tissue samples from RNA extraction to microarray analysis still needs optimization.