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From RNA isolation to microarray analysis: Comparison of methods in FFPE tissues
Date
2016-01-01
Author
Belder, Nevin
Coskun, Oznur
ERDOĞAN, BEYZA DOĞANAY
İlk Dağ, Özlem
SAVAŞ, BERNA
ENSARİ, ARZU
ÖZDAĞ, Hilal
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Background: Genome-wide gene expression profiling analysis of FFPE tissue samples is indispensable for cancer research and provides the opportunity to evaluate links between molecular and clinical information, however, working with FFPE samples is challenging due to extensive cross-linking, fragmentation and limited quantities of nucleic acid. Thus, processing of FFPE tissue samples from RNA extraction to microarray analysis still needs optimization.
Subject Keywords
Pathology and Forensic Medicine
,
Cell Biology
URI
https://hdl.handle.net/11511/40833
Journal
PATHOLOGY RESEARCH AND PRACTICE
DOI
https://doi.org/10.1016/j.prp.2015.11.008
Collections
Department of Statistics, Article
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N. Belder et al., “From RNA isolation to microarray analysis: Comparison of methods in FFPE tissues,”
PATHOLOGY RESEARCH AND PRACTICE
, pp. 678–685, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40833.