Chemoselective Immobilization of Proteins by Microcontact Printing and Bio-orthogonal Click Reactions

Date

2013-12-16

Author

Tolstyka, Zachary P.
Richardson, Wade
Bat, Erhan
Stevens, Caitlin J.
Parra, Dayanara P.
Dozier, Jonathan K.
Distefano, Mark D.
Dunn, Bruce
Maynard, Heather D.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

14
views

16
downloads

Cite This

Herein, a combination of microcontact printing of functionalized alkanethiols and site-specific modification of proteins is utilized to chemoselectively immobilize proteins onto gold surfaces, either by oxime- or copper-catalyzed alkyne-azide click chemistry. Two molecules capable of click reactions were synthesized, an aminooxy-functionalized alkanethiol and an azide-functionalized alkanethiol, and self-assembled monolayer (SAM) formation on gold was confirmed by IR spectroscopy. The alkanethiols were then individually patterned onto gold surfaces by microcontact printing. Site-specifically modified proteinshorse heart myoglobin (HHMb) containing an N-terminal -oxoamide and a red fluorescent protein (mCherry-CVIA) with a C-terminal alkynewere immobilized by incubation onto respective stamped functionalized alkanethiol patterns. Pattern formation was confirmed by fluorescence microscopy.

Subject Keywords

Organic Chemistry, Molecular Medicine, Biochemistry, Molecular Biology

URI

https://hdl.handle.net/11511/41903

Collections

Department of Chemical Engineering, Article