Space-resolved tissue analysis by solid-phase microextraction coupled to high-resolution mass spectrometry via desorption electrospray ionization

Date

2019-08-06

Author

Lendor, Sofia
Gómez-Ríos, Germán Augusto
Boyacı, Ezel
Vander Heide, Harmen
Pawliszyn, Janusz

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

58
views

0
downloads

It is hard to overstate the tremendous utility of desorption electrospray ionization (DESI) and its various configurations for rapid and high-throughput analyses or spatially resolved imaging of heterogeneous systems. However, there have been few attempts to employ this technique in spatially resolved mode with solid substrates featuring extractive and analyte-enrichment properties. This study documents the development of a platform that combines solid-phase microextraction (SPME) with desorption electrospray ionization mass spectrometry (DESI-MS) for unidimensional investigation of the heterogeneous distribution of compounds in semisolid systems (i.e., depth profiling across the fiber axis), with the ultimate end of employing it for brain tissue analysis. To this end, a DESI interface and a custom holder accommodating SPME probes were built in house, with the latter contributing to reduction of mechanical sources of signal instability. The system was evaluated through the quantitative SPME na reconstruction of the laminar and radial concentration gradients of xenobiotics introduced in multilayer gel arrangements and surrogate brain tissue models. Good quantitative capability was achieved by employing a strategy that combined signal correction via preloading internal standard onto SPME fibers and signal integration in scan-by-scan mode. The proposed technique's suitability for characterizing more complex systems, such as rat brains ex vivo, was also evaluated. The proposed approach allows for fast and noninvasive probing of three-dimensional objects without the need for their slicing, and the space-resolved mode reduces the number of required probe insertions, allowing in vivo applications. We foresee suitability of this setup for examining the spatial patterns of local drug release in the brain and the extent of the resultant physiological responses.

Subject Keywords

Analytical Chemistry

URI

https://hdl.handle.net/11511/42149

Journal

Analytical Chemistry

DOI

https://doi.org/10.1021/acs.analchem.9b02157

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Spectroscopic intensities as measures of order parameter close to order-disorder transitions Yurtseven, Hasan Hamit (Springer Science and Business Media LLC, 1988-01-01) The infrared and Raman intensities can be related to the order parameter close to order-disorder phase transitions in crystal systems. In the present study this relationship has been obtained and compared with our experimental results for ammonium halides. Our predictions give satisfactory agreement with the observations in these crystals. It is proposed that the FTIR techniques can be appropriately used to evaluating the order parameter in various crystal systems.
Hydrogen sulfide determination by solid surface luminescence Eroglu, AE; Volkan, Mürvet; Bayramh, E; Ataman, Osman Yavuz; Mark, HB (1996-07-01) In the analytical system suggested, atmospheric hydrogen sulfide reacts with the surface of a filter paper treated with aqueous cadmium chloride and yields a luminescent species whose intensity can be correlated with the analyte concentration in ambient air. It was shown that the luminescent species are CdS solid particles which were formed in a well defined size. The paper luminescence was also tried on polymeric surfaces; polyethyleneoxide, polyvinyl alcohol, ethylcellulose and carboxymethylcellulose were...
Preconcentration of volatile elements on quartz surface prior to determination by atomic spectrometry Korkmaz, Deniz; Ataman, Osman Yavuz; Department of Chemistry (2004) Hydride generation technique is frequently used for the detection of elements as As, Se, Sb, Sn, Bi, Ge, Te and Pb that form volatile hydrides in solution using a reductant. In this study, a novel quartz trap for on-line preconcentration of volatile analyte species was designed. Pb, Sb and Cd were selected as analyte elements and chemical vapour generation technique was employed for generation of their volatile species in flow systems. The trapping medium was formed by external heating of either the inlet a...
Pressure dependence of the Raman frequencies for the modes I, II and III at constant temperatures in phase II of benzene Ozdemir, H.; Yurtseven, Hasan Hamit (Elsevier BV, 2015-01-25) We study here the pressure dependence of the Raman frequencies for different modes in phase II of benzene at constant temperatures. By using the experimental data for the volume, we calculate the Raman frequency as a function of pressure at constant temperatures through the isothermal mode Gruneisen parameter for the modes studied. Our calculations show that the Raman frequencies predicted, agree well with the observed frequencies for the modes in phase II of benzene. The isothermal mode Gruneisen parameter...
Vibrational frequencies studied as a function of pressure in phase II of solid benzene Yurtseven, Hasan Hamit; Kilit, E. (Elsevier BV, 2011-05-03) The pressure dependence of the vibrational frequencies of six Raman modes is analyzed in phase II of solid benzene. The frequency shifts 1/nu(partial derivative nu/partial derivative P)(T) of those Raman modes are deduced from this analysis and they are related to the isothermal compressibility kappa(T)= -(1/V)(partial derivative V/partial derivative P)(T) through a constant isothermal mode Gruneisen parameter gamma(T) in phase II of solid benzene. The kappa(T) values predicted here are compared with the ka...

Citation Formats

S. Lendor, G. A. Gómez-Ríos, E. Boyacı, H. Vander Heide, and J. Pawliszyn, “Space-resolved tissue analysis by solid-phase microextraction coupled to high-resolution mass spectrometry via desorption electrospray ionization,” Analytical Chemistry, pp. 10141–10148, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42149.