Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis

2015-10-01
Sahin, Burcu
Ozturk, Sibel
Çalık, Pınar
Ozdamar, Tuncer H.
Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC (-)) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and (NH4)(2)HPO4 at two pre-determined specific growth rates, A mu (0) = 0.10 and 0.17 h(-1). Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, (NH4)(2)HPO4, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, A mu (0) = 0.10, 0.17, and 0.25 h(-1). At all the strategies applied, transition cultivation time from batch to fed-batch operation was t (T) = 4 h. The highest rhGH concentration was obtained as C (rhGH) = 0.5 g L-1 with semi-defined medium-based feeding strategy designed with A mu (0) = 0.25 h(-1) using feed substrate stock solution containing 200 g L-1 glucose, 117 g L-1 (NH4)(2)HPO4, 100 g L-1 peptone, and 5 mL L-1 PTM1 at t = 22 h when the cell concentration reached to C (X) = 8.29 g L-1. The overall product and cell yields on glucose were obtained as = 7.21 mg g(-1) and = 0.12 g g(-1), respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis.

Citation Formats
B. Sahin, S. Ozturk, P. Çalık, and T. H. Ozdamar, “Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis,” BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 38, no. 10, pp. 1855–1865, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42571.