Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis

Date

2015-10-01

Author

Sahin, Burcu
Ozturk, Sibel
Çalık, Pınar
Ozdamar, Tuncer H.

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Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC (-)) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and (NH4)(2)HPO4 at two pre-determined specific growth rates, A mu (0) = 0.10 and 0.17 h(-1). Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, (NH4)(2)HPO4, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, A mu (0) = 0.10, 0.17, and 0.25 h(-1). At all the strategies applied, transition cultivation time from batch to fed-batch operation was t (T) = 4 h. The highest rhGH concentration was obtained as C (rhGH) = 0.5 g L-1 with semi-defined medium-based feeding strategy designed with A mu (0) = 0.25 h(-1) using feed substrate stock solution containing 200 g L-1 glucose, 117 g L-1 (NH4)(2)HPO4, 100 g L-1 peptone, and 5 mL L-1 PTM1 at t = 22 h when the cell concentration reached to C (X) = 8.29 g L-1. The overall product and cell yields on glucose were obtained as = 7.21 mg g(-1) and = 0.12 g g(-1), respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis.

Subject Keywords

Fed-Batch Operation, Exponential Feeding, Glucose, Semi-Defined Medium, Human Growth Hormone, Bacillus Subtilis

URI

https://hdl.handle.net/11511/42571

Journal

BIOPROCESS AND BIOSYSTEMS ENGINEERING

DOI

https://doi.org/10.1007/s00449-015-1426-3

Collections

Department of Chemical Engineering, Article

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Citation Formats

B. Sahin, S. Ozturk, P. Çalık, and T. H. Ozdamar, “Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis,” BIOPROCESS AND BIOSYSTEMS ENGINEERING, pp. 1855–1865, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42571.