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Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis
Date
2009-01-01
Author
ÖZDAMAR, HASAN TUNÇER
Sentuerk, Birguel
Yilmaz, Oezge Deniz
Calik, Guezide
Celik, Eda
Çalık, Pınar
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We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis and secretion, producing native form of human growth hormone (hGH) from Bacillus subtilis. A hybrid-gene of two DNA fragments, i.e., signal (pre-) DNA sequence of B. licheniformis serine alkaline protease gene (subC) and cDNA encoding hGH, were cloned into pMK4 and expressed under deg-promoter in B. subtilis. Recombinant-hGH (rhGH) produced by B. subtilis carrying pMK4::pre(subC)::hGH was secreted. N-terminal sequence and mass spectrometry analyses of rhGH confirm the mature hGH sequence, and indicate that the signal peptide was properly processed by B. subtilis signal-peptidase. The highest rhGH concentration was obtained at t = 32 h as C(rhGH) = 70 mg L(-1) with a product yield on substrate Y(rhGHIS) = 9 g kg(-1), in a glucose based defined medium. Fermentation characteristics and influence of hGH gene on the rhGH production were investigated by comparing B. subtilis carrying pMK4::pre(subC)::hGH with that of carrying merely pMK4. Excreted organic-acid concentrations were higher by B. subtilis carrying pMK4::pre(subC)::hGH, whereas excreted amino-acid concentrations were higher by B. subtilis carrying pMK4. The approach developed is expected to be applicable to the design of expression systems Jar heterologous protein production front Bacillus species. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 75-84, 2009
Subject Keywords
Bacillus
,
Recombinant
,
Protein
,
Human growth hormone
,
DegQ
,
Signal peptide
,
Expression
,
Secretion
,
MALDI-MS
,
Fermenttin
URI
https://hdl.handle.net/11511/48834
Journal
BIOTECHNOLOGY PROGRESS
DOI
https://doi.org/10.1021/bp.81
Collections
Department of Chemical Engineering, Article
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H. T. ÖZDAMAR, B. Sentuerk, O. D. Yilmaz, G. Calik, E. Celik, and P. Çalık, “Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis,”
BIOTECHNOLOGY PROGRESS
, pp. 75–84, 2009, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48834.