Effects of spray drying temperature and additives on the stability of serine alkaline protease powders

Date

2006-01-01

Author

Namaldi, Aysegul
Çalık, Pınar
Uludağ, Yusuf

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

218
views

0
downloads

In this study, after production by recombinant Bacillus subtilis (BGSC-1A751), carrying pHV1431:: subc gene in the complex medium and separation of solids from the fermentation broth, serine alkaline protease (SAP) was dried in order to investigate the stabilization during spray drying and subsequent storage. The effect of air inlet temperature of the spray dryer between T = 70 and 130 degrees C and the effect of protective additives, glucose and maltodextrin, at 0-2% (w/v) on SAP activity during spray drying and storage stability of obtained SAP powders at 4 degrees C for a long period (6 months) were evaluated. Increasing drying air inlet temperature generally resulted in an increase in activity loss; moreover, higher absorbance peaks observed at wave number 1061 cm(-1) of the IR spectrums when drying temperature is increased indicates the structural change in the SAP molecule. In most cases presence of additives provided higher activities both after drying and during storage period compared to no additive case. Drying the enzyme with 1% (w/v) glucose at T =110 degrees C resulted in the highest enzyme activity after drying and storage processes.

Subject Keywords

Storage stability, Spray drying, Serine alkaline protease, Protective additives, Activity loss

URI

https://hdl.handle.net/11511/42617

Journal

DRYING TECHNOLOGY

DOI

https://doi.org/10.1080/07373930600961108

Collections

Department of Chemical Engineering, Article

Suggestions

OpenMETU
Core

Influence of pH conditions on metabolic regulations in serine alkaline protease production by Bacillus licheniformis Çalık, Pınar; Calik, G; Ozdamar, TH (2002-10-03) The effects of the controlled and uncontrolled pH conditions, as well as of the value of initial pH in the range of 7-7.5, on serine alkaline protease (SAP) production by Bacillus licheniformis were investigated on a defined medium with the single carbon source glucose in batch bioreactors. Besides uncontrolled pH operations, growth phase, production phase, and entire process control strategies were also applied. The concentrations of the product (SAP) and by-products, i.e. neutral protease, amylase, amino ...
Effects of bond-coatings on plasma sprayed calciumphosphate coatings Oktar, FN; Goller, G; Yetmez, M; Toykan, D (2003-01-01) In this study the effects of bond-coatings on various calcium phosphate coatings were studied. From human teeth dentine (DFA) and enamel derived fluorapatite (EFA) plasma powders were sprayed on titanium with and without a bond-coating. As control group original hydroxyapatite (HA) powder for biomedical spraying purposes was also used on titanium with and without a bond-coating. Tensile strength tests were performed according to the F-633 ASTM standards. It was observed that bond-coatings had significantly ...
Effect of synthesis media pH and gel separation technique on properties of copper incorporated SBA-15 catalyst Akti, Filiz; Balci, Suna; Doğu, Timur (2019-10-01) Cu-SBA-15 catalysts were synthesized hydrothermally under acidic and slightly higher acidic conditions via solid separation by either gel filtration or evaporation. XRD (X-ray diffraction) patterns and HRTEM (high resolution transmission electron microscopy) images showed that metal loading caused little changes in the ordered structure of SBA-15. Characteristic structural SBA-15's bonds and acid sites were observed in FTIR (Fourier transform infrared) spectrums and synthesis under high pH was created chang...
Effects of surface pretreatments and coating period on hydroxyapatite coating of Ti6Al4V alloy HACIOĞLU, TUĞÇE; Evis, Zafer; Tezcaner, Ayşen; Aydınol, Mehmet Kadri (2020-06-01) In this study, Ti6Al4V plates were gone through three steps of pretreatment process (sandblasting + acid etching + preheating) followed by biomimetic coating of pure hydroxyapatites (HAp) on pretreated substrate. Pretreated substrates were examined by SEM, EDS, AFM, FTIR, and XRD; meanwhile, prepared coatings were examined by SEM, EDS, FTIR, XRD, XPS, scratch test, and in vitro cell studies (SaOS-2 cell line). Prepared coatings were also investigated in terms of antibacterial property by determining the sur...
Effects of psyllium and cellulose fibres on thermal, structural, and in vitro digestion behaviour of wheat starch Atac, Leyla Elif; Şensoy, İlkay (2021-10-01) The study investigated the effects of adding different types of fibre (psyllium and cellulose) on in vitro digestion behaviour of wheat starch with its thermal and structural properties. Psyllium and cellulose fibres interfered with the wheat starch differently. Psyllium fibre hindered starch gelatinisation, restricted the loss of starch crystallinity, and decreased the accessibility of enzymes to starch. In contrast, cellulose fibre had no significant effect on gelatinisation and loss of crystallinity but ...

Citation Formats

A. Namaldi, P. Çalık, and Y. Uludağ, “Effects of spray drying temperature and additives on the stability of serine alkaline protease powders,” DRYING TECHNOLOGY, pp. 1495–1500, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42617.