Influence of boron toxicity on global expression profiles in wheat (Triticum aestivum L.)

Date

2012-09-23

Author

Kayihan, C.
Oz, M. T.
EYİDOĞAN, FÜSUN
YÜCEL, MUSTAFA
Öktem, Hüseyin Avni

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

147
views

0
downloads

Excess boron (B) is known to negatively affect plant growth. However, the molecular mechanism of toxicity in plants is not satisfactory elucidated. In this study, global expression differences in wheat (Triticum aestivum L. cv. Atay-85 and Bolal-2973) leaves and roots under B toxicity were investigated using DNA microarrays. Eight day old aseptically grown seedlings were subjected to 5 mM H3BO3 for 5 days. Total RNAs from leaf or root tissues were isolated and checked by Bioanalyzer 2100. Expression profiles were determined with Wheat GeneChip. Among 61127 transcripts – each represented with a probe set on the GeneChip – fold change of at least 2 and p value of at most 0.05 was considered as an indication of significant alteration in expression. Compared to unstressed control (10 μM H3BO3) conditions, B toxicity resulted in significant regulation of more than 1000 transcripts in leaf and root tissues of both cultivars. Furthermore, the numbers of genes significantly expressed in Atay-85 were higher than those in Bolal-2973. Transcripts altered under B toxicity included genes coding for transcription factors, peroxidases, ribosomal proteins, and hydrolases and functioning in biosynthesis of phenylpropanoids. Responsive genes determined in this study will provide insight into successive studies on molecular mechanism of tolerance to B toxicity in wheat.

Subject Keywords

Antibacterial, Lactic acid bacteria, Escherichia coli, Lactobacillus delbrueckii

URI

https://hdl.handle.net/11511/42629

Journal

NEW BIOTECHNOLOGY

DOI

https://doi.org/10.1016/j.nbt.2012.08.353

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Effect of boron and iron stress on phytosiderophore production in barley İnce, Yetkin Çaka; Yücel, Ayşe Meral; Department of Biology (2015) Boron and iron are essential nutrients for growth and development of higher plants. Deficiency and toxicity of them cause significant changes in physiological, cellular and molecular make-up of plants. Iron uptake in barley is mainly achieved by production and release of phytosiderophores to the environment. On the other hand, production of phytosiderophores are affected by concentrations of boron and iron. Four enzymes which function in phytosiderophore hydroxymugineic acid (HMA) production namely NAS, NAA...
Effects of plant size on the growth of the submersed macrophyteVallisneria spinulosaSZYan at different light intensities: implications for lake restoration Yuan, Guixiang; Fu, Hui; Zhang, Meihong; Lou, Qian; Dai, Taotao; Jeppesen, Erik (Springer Science and Business Media LLC, 2020-10-01) The growth and reproduction of plants are highly size-dependent and markedly affected by light intensity.Vallisneria spinulosaS.Z.Yan is a common submersed macrophyte and is widely used in current lake restoration projects. The size of plants to be used to get optimal restoration results is not known, however, and may vary with water clarity. In this study, the effects of different plant sizes (large, medium and small, i.e. approximately 2.40 g, 1.01 g and 0.27 g per plant) on the growth and reproduction of...
INFLUENCE OF HEAVY METALS ON MICROBIAL GROWTH KINETICS INCLUDING LAG TIME: MATHEMATICAL MODELING AND EXPERIMENTAL VERIFICATION Şengör, Sema Sevinç; Gikas, Petros; Ginn, Timothy R.; Peyton, Brent; Sani, Rajesh K.; Spycher, Nicolas F. (2009-10-01) Heavy metals can significantly affect the kinetics of substrate biodegradation and microbial growth, including lag times and specific growth rates. A model to describe microbial metabolic lag as a function of the history of substrate concentration has been previously described by Wood et al. (Water Resour Res 31: 553-563) and Ginn (Water Resour Res 35: 1395-1408). In the present study, this model is extended by including the effect of heavy metals on metabolic lag by developing an inhibitor-dependent functi...
Effect of sodium carbonate on carbothermic formation of hexagonal boron nitride Akyıldız, Uğur; Sevinç, Naci; Department of Metallurgical and Materials Engineering (2010) Effect of Na2CO3 on formation of hexagonal boron nitride (h-BN) by carbothermic method has been studied by subjecting B2O3-C and Na2CO3-added B2O3-C mixtures to N2 (g) atmosphere. Na2CO3 amount in the mixtures was changed in the range of 0-40 wt. %. Time and temperature were used as experimental variables. Reaction products were analyzed by XRD and scanning electron microscope. Na2CO3 was found to increase both the amount and the particle size of h-BN similar to CaCO3 [1]. Na2CO3 was found to be less effect...
Effect of different stresses on trehalose levels in Rhizopus oryzae Uyar, Ebru Ozer; Hamamcı, Haluk; TÜRKEL, SEZAİ (2010-08-01) Rhizopus oryzae accumulates and degrades trehalose in response to environmental stress conditions such as heat, osmotic stress, nitrogen starvation and pH. When heat stress was applied to R. oryzae, the trehalose content of the cells was increased from 0.9 to 4.8 mg/g dw and when the stress was relieved it decreased back to 1.1 mg/g dw. Under osmotic stress and nitrogen starvation, trehalose content was increased by 3.5 and 3 fold, respectively. The decrease in external pH increased trehalose level up to 2....

Citation Formats

C. Kayihan, M. T. Oz, F. EYİDOĞAN, M. YÜCEL, and H. A. Öktem, “Influence of boron toxicity on global expression profiles in wheat (Triticum aestivum L.),” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42629.