Influence of boron toxicity on global expression profiles in wheat (Triticum aestivum L.)

Date

2012-09-23

Author

Kayihan, C.
Oz, M. T.
EYİDOĞAN, FÜSUN
YÜCEL, MUSTAFA
Öktem, Hüseyin Avni

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Excess boron (B) is known to negatively affect plant growth. However, the molecular mechanism of toxicity in plants is not satisfactory elucidated. In this study, global expression differences in wheat (Triticum aestivum L. cv. Atay-85 and Bolal-2973) leaves and roots under B toxicity were investigated using DNA microarrays. Eight day old aseptically grown seedlings were subjected to 5 mM H3BO3 for 5 days. Total RNAs from leaf or root tissues were isolated and checked by Bioanalyzer 2100. Expression profiles were determined with Wheat GeneChip. Among 61127 transcripts – each represented with a probe set on the GeneChip – fold change of at least 2 and p value of at most 0.05 was considered as an indication of significant alteration in expression. Compared to unstressed control (10 μM H3BO3) conditions, B toxicity resulted in significant regulation of more than 1000 transcripts in leaf and root tissues of both cultivars. Furthermore, the numbers of genes significantly expressed in Atay-85 were higher than those in Bolal-2973. Transcripts altered under B toxicity included genes coding for transcription factors, peroxidases, ribosomal proteins, and hydrolases and functioning in biosynthesis of phenylpropanoids. Responsive genes determined in this study will provide insight into successive studies on molecular mechanism of tolerance to B toxicity in wheat.

Subject Keywords

Antibacterial, Lactic acid bacteria, Escherichia coli, Lactobacillus delbrueckii

URI

https://hdl.handle.net/11511/42629

Journal

NEW BIOTECHNOLOGY

DOI

https://doi.org/10.1016/j.nbt.2012.08.353

Collections

Department of Biology, Article

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Citation Formats

C. Kayihan, M. T. Oz, F. EYİDOĞAN, M. YÜCEL, and H. A. Öktem, “Influence of boron toxicity on global expression profiles in wheat (Triticum aestivum L.),” NEW BIOTECHNOLOGY, pp. 0–0, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42629.