Microarray analysis of high light intensity stress on hydrogen production metabolism of Rhodobacter capsulatus

Gurgan, Muazzez
Koku, Harun
Yucel, Meral
Biohydrogen obtained from purple non sulfur bacteria (PNSB) is an environmentally friendly alternative for hydrogen production. PNSB can be employed in large scale outdoor photobioreactors to produce hydrogen by photofermentation with sunlight as the light source. In external environmental conditions, however, bacteria can experience stress due to high light intensities, which can inhibit or slow down hydrogen production. Previous studies with other PNSB showed varying responses to light intensities (above 4000 lux), in some cases improving, and in others adversely affecting hydrogen production.


Pilot-scale outdoor photofermentative hydrogen production from molasses using pH control
Oflaz, F. Betül; Koku, Harun (Elsevier BV, 2020-01-01)
© 2020 Hydrogen Energy Publications LLCThe duration of photofermentative hydrogen production from sugar-based nutrients is limited by gradual acidification caused by the production of organic acids, leading to suboptimal pH. To address this issue, a custom pH control system was built and installed on a 20 L tubular photobioreactor, and operated under outdoor conditions. Long-term, single-stage hydrogen production from molasses was achieved using the purple non-sulfur bacterium, Rhodobacter capsulatus. The r...
Transcriptome analysis of the effects of light and dark cycle on hydrogen production metabolism of Rhodobacter capsulatus DSM1710
Gürgan, Muazzez; Koku, Harun; Eroglu, Inci; Yücel, Meral (Elsevier BV, 2020-01-01)
© 2020 Hydrogen Energy Publications LLCPhotosynthetic purple non sulfur bacteria can be utilized in outdoor photobioreactors for sustainable large-scale biohydrogen production. However, the diurnal cycle under natural outdoor conditions affects hydrogen productivity and yield. Here, we investigated the effect of light and dark cycles on the hydrogen production metabolism of Rhodobacter capsulatus DSM1710 using custom-designed Affymetrix Gene Chip technology. R. capsulatus was grown by cyclic illumination on...
Microarray analysis of the effects of light intensity on hydrogen production metabolism of rhodobacter capsulatus
Gürgan Eser, Muazzez; Yücel, Ayşe Meral; Koku, Harun; Department of Biology (2017)
Biohydrogen generated by purple non-sulfur bacteria is a clean and renewable method of hydrogen production. It can be achieved in outdoor phototobioreactors using the natural sun light in lab to pilot scales. Light is one of the most important parameter affecting hydrogen production in the outdoor condition. Hydrogen productivity may decrease upon light intensity stress by sun light and the diurnal cycle in outdoor conditions. It is important to understand the metabolic response of these bacteria to varying...
Parametric Study of Carbon Dioxide Sequestration in Deep Saline Aquifers
Basbug, B.; Gumrah, F. (Informa UK Limited, 2009-01-01)
CO2 sequestration in a deep saline aquifer was simulated using a compositional commercial simulator based on a single-well aquifer model, which was developed to analyze the injection and storage process of CO2 under supercritical and gaseous conditions for long periods of time. The influence of controlling parameters including vertical to horizontal permeability ratio, aquifer pressure, injection rate on the isothermal injection, and sequestration processes were investigated and the sensitivity analysis cor...
Design of an outdoor stacked - tubular reactor for biological hydrogen production
KAYAHAN, Emine; Eroglu, Inci; Koku, Harun (Elsevier BV, 2016-11-02)
Photofermentation is one alternative to produce hydrogen sustainably. The photobioreactor design is of crucial importance for an economically feasible operation, and an optimal design should provide uniform velocity and light distribution, low pressure drop, low gas permeability and efficient gas-liquid separation. A glass, stacked tubular bioreactor aimed at satisfying these criteria has been designed for outdoor photofermentative hydrogen production by purple non sulfur bacteria. The design consists of 4 ...
Citation Formats
M. Gurgan, H. Koku, İ. EROĞLU, and M. Yucel, “Microarray analysis of high light intensity stress on hydrogen production metabolism of Rhodobacter capsulatus,” INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, pp. 3516–3523, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42637.