Application of in situ Solid-Phase Microextraction on Mediterranean Sponges for Untargeted Exometabolome Screening and Environmental Monitoring

Download

index.pdf

Date

2019-10-11

Author

Bojko, Barbara
Onat, Bora
Boyacı, Ezel
Psillakis, Eleftheria
Dailianis, Thanos
Pawliszyn, Janusz

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

172
views

76
downloads

In marine ecosystems, sponges are ubiquitous sessile organisms that contain a wide range of specialized metabolites. These metabolites point to a diverse range of biochemical pathways. Some of these compounds are biomarkers that indicate the presence of bacteria that form symbiotic relationships with a host sponge (microsymbionts). Sponges hold considerable promise as bioindicators for seawater quality monitoring, as they are exposed to, and accumulate, significant levels of anthropogenic contamination in coastal areas. Solid-phase microextraction (SPME) is a low-invasive and non-exhaustive technique that combines sampling and extraction into a single step and offers the added benefit of biocompatible extraction phases. We used different types of SPME devices to extract exometabolites from sponges (genus: Sarcotragus) in situ. Following extraction, the samples were analyzed via GC- and LC-MS in order to verify the presence of compounds associated with quorum sensing, as well as to examine the metabolism of organic pollutants, such as monocyclic aromatic hydrocarbons (MAHs), polycyclic aromatic hydrocarbons (PAHs), pesticides, and other bioactive compounds in an untargeted format. As the results demonstrate, when the extracted metabolites are compared with the background controls, SPME offers a non-exhaustive approach that can be used in the field to discover novel metabolites deriving from complex holobionts such as marine sponges.

Subject Keywords

Aquatic Science, Global and Planetary Change, Ocean Engineering, Oceanography, Water Science and Technology

URI

https://hdl.handle.net/11511/42785

Journal

Frontiers in Marine Science

DOI

https://doi.org/10.3389/fmars.2019.00632

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Decadal changes in zooplankton biomass, composition, and body mass in four shallow brackish lakes in Denmark subjected to varying degrees of eutrophication He, Hu; Jeppesen, Erik; Bruhn, Dan; Yde, Morten; Hansen, Jacob Kjerulf; Spanggaard, Lasse; Madsen, Niels; Liu, Wei; Sondergaard, Martin; Lauridsen, Torben L. (Informa UK Limited, 2020-04-01) During the past century, many brackish shallow lakes worldwide have become eutrophic. How the zooplankton have responded to this development is not well elucidated. Here, we analysed the decadal changes (from 1999-2000 to 2017-2018) in zooplankton biomass, body mass, and potential top-down control on phytoplankton during summer in 4 Danish shallow brackish lakes (Lund Fjord, Han Vejle, Selbjerg, and Glombak) subjected to varying degrees of eutrophication. Significant reductions of zooplankton biomass, body ...
Lake types and their definition: a case study from Denmark Sondergaard, Martin; Johansson, Liselotte S.; Levi, Eti E.; Lauridsen, Torben L.; Jeppesen, Erik (Informa UK Limited, 2020-04-01) In the EU Water Framework Directive, lakes are classified into different types primarily on the basis of morphological and chemical characteristics. Lake type definition is not trivial because the different lake types may require different nutrient loading reduction targets to achieve the goal of at least "good" ecological status. Using data from 770 Danish lakes, we illustrate some of the problems associated with classification. In Denmark, 16 potentially different lake types are defined using an alkalinit...
Energy-based top-down and bottom-up relationships between fish community energy demand or production and phytoplankton across lakes at a continental scale Bartrons, Mireia; Mehner, Thomas; Argillier, Christine; Beklioğlu, Meryem; Blabolil, Petr; Hesthagen, Trygve; Sweden, Kerstin Holmgren; Jeppesen, Erik; Krause, Teet; Podgornik, Samo; Volta, Pietro; Winfield, Ian J.; Brucet, Sandra (Wiley, 2020-04-01) Fish community feeding and production rates may differ between lakes despite similar fish biomass levels because of differences in size structure and local temperature. Therefore, across-lake comparisons of the strength and direction of top-down and bottom-up fish-phytoplankton relationships should consider these factors. We used the metabolic theory of ecology to calculate size- and temperature-corrected community energy demand (CEDom) and community production (CP) of omnivorous fishes in 227 European lake...
Parameterization of iron and manganese cycling in the Black Sea suboxic and anoxic environment Konovalov, S; Samodurov, A; Oguz, T; Ivanov, L (Elsevier BV, 2004-12-01) New and published data on the distribution and speciation of manganese and iron in seawater are analyzed to identify and parameterize major biogeochemical processes of their cycling within the suboxic (similar to15.6less than or equal tosigma(t)less than or equal tosimilar to16.2) and anoxic layers (sigma(t)less than or equal tosimilar to16.2) of the Black Sea. A steady-state transport-reaction model is applied to reveal layering and parameterize kinetics of redox and dissolution/precipitation processes. Pr...
Modelling phytoplankton succession on the Bering Sea shelf: role of climate influences and trophic interactions in generating Emiliania huxleyi blooms 1997-2000 Merico, A; Tyrrell, T; Lessard, EJ; Oguz, T; Stabeno, PJ; Zeeman, SI; Whitledge, TE (Elsevier BV, 2004-12-01) Several years of continuous physical and biological anomalies have been affecting the Bering Sea shelf ecosystem starting from 1997. Such anomalies reached their peak in a striking visual phenomenon: the first appearance in the area of bright waters caused by massive blooms of the coccolithophore Emiliania huxleyi (E huxleyi). This study is intended to provide an insight into the mechanisms of phytoplankton succession in the south-eastern part of the shelf during such years and addresses the causes of E. hu...

Citation Formats

B. Bojko, B. Onat, E. Boyacı, E. Psillakis, T. Dailianis, and J. Pawliszyn, “Application of in situ Solid-Phase Microextraction on Mediterranean Sponges for Untargeted Exometabolome Screening and Environmental Monitoring,” Frontiers in Marine Science, pp. 0–0, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42785.