Interleukin-15 acts as an immunological co-adjuvant for liposomal antigen in vivo

Date

1997-03-01

Author

Gürsel, Mayda

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The recently discovered interleukin-15 (IL-15) is known to bind to the receptor of interleukin-2 (IL-2) and to share several of the latter's immunological properties. In the present study, the first to our knowledge on IL-15 behaviour in vivo, we examined the possibility that IL-15 also shares the ability of IL-2 to enhance the immunological adjuvant property of liposomes by acting as a co-adjuvant. The cytokine and a model antigen (tetanus toroid) were either co-entrapped by the dehydration rehydration method into, or covalently co-linked by diazotization to the surface of the same liposomes, or entrapped in different liposome populations. Intramuscular immunization of CD-1 mice with a variety of IL-15 and toroid formulations revealed that IL-15 augments anti-toroid IgG (IgG(1,) IgG(2,) IgG(2b)) responses well above (up to ten-fold) those achieved with liposomal toroid alone (or with a mixture of free IL-15 and toroid) when the cytokine and the antigen are associated with the same vesicles but not when in different vesicle populations that were mixed before injection. Higher responses were observed for all three subclasses studied only with liposomes where IL-15 and antigen were accommodated on their surface. (C) 1997 Elsevier Science B.V.

Subject Keywords

Immunology, Immunology and Allergy

URI

https://hdl.handle.net/11511/43143

Journal

IMMUNOLOGY LETTERS

DOI

https://doi.org/10.1016/s0165-2478(97)02699-0

Collections

Department of Biology, Article

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Human peripheral blood cells differentially recognize and respond to two distinct CpG motifs Verthelyi, D; Ishii, KJ; Gürsel, Mayda; Takeshita, F; Klinman, DM (The American Association of Immunologists, 2001-02-15) Oligodeoxynucleotides (ODN) that contain unmethylated CpG dinucleotides trigger a strong innate immune response in vertebrates. CpG ODN show promise as vaccine adjuvants, anti-allergens, and immunoprotective agents in animal models. Their transition to clinical use requires the identification of motifs that are optimally stimulatory in humans. Analysis of hundreds of novel ODN resulted in the identification and characterization of two structurally distinct "clusters" of immunostimulatory CpG ODN, One cluste...
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CpG oligodeoxynucleotides protect normal and SIV-infected macaques from Leishmania infection. Verthelyi, D; Gürsel, Mayda; Kenney, RT; Lifson, JD; Liu, S; Mican, J; Klinman, DM (The American Association of Immunologists, 2003-05-01) Oligodeoxynucleotides containing CpG motifs (CpG ODNs) mimic microbial DNA and activate effectors of the innate immune response, which limits the spread of pathogens and promotes an adaptive immune response. CpG ODNs have been shown to protect mice from infection with intracellular pathogens. Unfortunately, CpG motifs that optimally stimulate humans are only weakly active in mice, mandating the use of nonhuman primates to monitor the activity and safety of "human" CpG ODNs in vivo. This study demonstrates t...
Potential role of phosphatidylinositol 3 kinase, rather than DNA-dependent protein kinase, in CpG DNA-induced immune activation. Ishii, KJ; Takeshita, F; Gursel, I; Gürsel, Mayda; Conover, J; Nussenzweig, A; Klinman, DM (Rockefeller University Press, 2002-07-15) Unmethylated CpG motifs present in bacterial DNA stimulate a strong innate immune response. There is evidence that DNA-dependent protein kinase (DNA-PK) mediates CpG signaling. Specifically, wortmannin (an inhibitor of phosphatidylinositol 3 kinase [PI3]-kinases including DNA-PK) interferes with CpG-dependent cell activation, and DNA-PK knockout (KO) mice fail to respond to CpG stimulation. Current studies establish that wortmannin actually inhibits the uptake and colocalization of CpG DNA with toll-like re...

Citation Formats

M. Gürsel, “Interleukin-15 acts as an immunological co-adjuvant for liposomal antigen in vivo,” IMMUNOLOGY LETTERS, pp. 161–165, 1997, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43143.