Molecular evaluation and antimicrobial susceptibility testing of Escherichia coli isolates from food products in Turkey

Kyere, Emmanuel Owusu
Bulut, Ece
Soyer, Yeşim
Some strains of Escherichia coli can be important food borne pathogens. Characterization and antimicrobial resistance testing of 28 E. coli isolates from random food samples obtained in Van, Turkey were performed. Primers for 6 indicator genes (fliC, stx1, stx2, eae, hlyA, and rfbE) for shiga toxin-producing E. coli and 5 indicator genes for each pathogroup (bfpA, aggR, ipaH, daaD, st, and lt) were used. E. coli isolates were also typed using pulsed field gel electrophoresis with the XbaI restriction enzyme. Antimicrobial susceptibility of E. coli isolates was determined using the disk diffusion method for 17 antimicrobials. E. coli isolates were non-pathogenic strains represented by 25 distinguishable PFGE patterns. Antimicrobial susceptibility testing revealed that more than 40% of the E. coli isolates showed resistance to ampicillin, sulphafurazole, and tetracycline. Antimicrobial susceptibility of commensal E. coli should be monitored because these bacteria are becoming reservoirs of antimicrobial resistance genes.


Assessing genetic heterogeneity within bacterial species isolated from gastrointestinal and environmental samples: How many isolates does it take?
Dopfer, D.; Buist, W.; Soyer, Yeşim; Munoz, M. A.; Zadoks, R. N.; Geue, L.; Engel, B. (American Society for Microbiology, 2008-06-01)
Strain typing of bacterial isolates is increasingly used to identify sources of infection or product contamination and to elucidate routes of transmission of pathogens or spoilage organisms. Usually, the number of bacterial isolates belonging to the same species that is analyzed per sample is determined by convention, convenience, laboratory capacity, or financial resources. Statistical considerations and knowledge of the heterogeneity of bacterial populations in various sources can be used to determine the...
Biodegradation of the Allelopathic Chemical Pterostilbene by a Sphingobium sp. Strain from the Peanut Rhizosphere
Yu, Ri-Qing; Kurt, Zöhre; He, Fei; Spain, Jim C. (American Society for Microbiology, 2019-03-01)
Many plants produce allelopathic chemicals, such as stilbenes, to inhibit pathogenic fungi. The degradation of allelopathic compounds by bacteria associated with the plants would limit their effectiveness, but little is known about the extent of biodegradation or the bacteria involved. Screening of tissues and rhizosphere of peanut (Arachis hypogaea) plants revealed substantial enrichment of bacteria able to grow on resveratrol and pterostilbene, the most common stilbenes produced by the plants. Investigati...
Current applications of exopolysaccharides from lactic acid bacteria in the development of food active edible packaging
Moradi, Mehran; Guimarães, Jonas T; Şahin, Serpil (Elsevier BV, 2021-08-01)
The lactic acid bacteria (LAB) are usually recognized as safe for consumption and comprise several genera with different technological and health-promoting potential for food applications, including probiotic characteristics. Their exopolysaccharides (EPS) have interesting film-forming properties and may be used to produce edible packaging, as a structural enhancer, a bioactive agent or probiotic carrier in edible films and coatings. In these days, there is a specific demand for food products with reduced a...
Molecular evaluation and antimicrobial susceptibility testing of escherichia coli isolates collected from various food products in Turkey/
Kyere, Emmanuel Owusu; Soyer, Yeşim; Avşaroğlu, Dilek; Department of Biotechnology (2014)
Even though Escherichia coli (E. coli) is part of the intestinal microflora of healthy human beings and warm blooded animals, some strains of E. coli can be important food borne pathogens that can cause a wide spectrum of diseases, ranging from self-limiting to life threatening intestinal and extra-intestinal illnesses. Pathogenic E. coli that affect the intestines of humans have been grouped into six main pathotypes: Shiga-toxin-producing E. coli (STEC); of which enterohaemorrhagic E. coli (EHEC) is a path...
Killer toxin of Pichia anomala NCYC 432; purification, characterization and its exo-beta-1,3-glucanase activity
Izgu, Fatih; Altinbay, Demet; Acun, Tolga (Elsevier BV, 2006-08-02)
Pichia anomala NCYC 432 secretes a killer toxin which is inhibitory to a variety of yeasts including pathogenic Candida spp. The killer toxin in the culture supernatant was concentrated by ultratiltration and purified to homogenity by two successive gel filtration chromatographies with a TSK G2000SW column. Biochemical characterization of the toxin showed that it is a glycosylated protein with a molecular mass of 47 kDa and pI values of 3.4 and 3.7. The toxin showed high stability at pH values between 3 and...
Citation Formats
E. O. Kyere, E. Bulut, M. D. AVŞAROĞLU ERKAN, and Y. Soyer, “Molecular evaluation and antimicrobial susceptibility testing of Escherichia coli isolates from food products in Turkey,” FOOD SCIENCE AND BIOTECHNOLOGY, pp. 1001–1009, 2015, Accessed: 00, 2020. [Online]. Available: