Killer toxin of Pichia anomala NCYC 432; purification, characterization and its exo-beta-1,3-glucanase activity

Izgu, Fatih
Altinbay, Demet
Acun, Tolga
Pichia anomala NCYC 432 secretes a killer toxin which is inhibitory to a variety of yeasts including pathogenic Candida spp. The killer toxin in the culture supernatant was concentrated by ultratiltration and purified to homogenity by two successive gel filtration chromatographies with a TSK G2000SW column. Biochemical characterization of the toxin showed that it is a glycosylated protein with a molecular mass of 47 kDa and pI values of 3.4 and 3.7. The toxin showed high stability at pH values between 3 and 5.5 and up to 37 degrees C. Its N-terminal amino acid sequencing yielded the sequence GDYWDYQNDKIR which is 100% identical with that of mature exo-beta-1,3-glucanase (accession no. AJ222862) of P. anomala strain K. The toxin displayed high activity against laminarin thus showing a beta-glucanase activity. The Michaelis constants K-m and V-max for laminarin hydrolysis were 0.3 mg ml(-1) and 350 mu mol min(-1) mg(-1). (c) 2005 Elsevier Inc. All rights reserved.


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Citation Formats
F. Izgu, D. Altinbay, and T. Acun, “Killer toxin of Pichia anomala NCYC 432; purification, characterization and its exo-beta-1,3-glucanase activity,” ENZYME AND MICROBIAL TECHNOLOGY, pp. 669–676, 2006, Accessed: 00, 2020. [Online]. Available: