Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Characterization of sorting nexin 3 (SNX3) function
Download
index.pdf
Date
2020
Author
Öyken, Merve
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
111
views
35
downloads
Cite This
Sorting nexin 3 (SNX3) belongs to the sorting nexins family involved in endocytic trafficking network. SNX3 only has a PX domain, which facilitates binding to the phosphatidyl inositol-3-phosphate on endosomes and has a role in formation of the retromer complex on early endosomes. Endocytosed receptors go through internalization and recycle back to plasma membrane, Trans-Golgi Network or alternatively are degraded in the lysosomes. Earlier, we found that SNX3 messenger RNA (mRNA) is upregulated via alternative polyadenylation in breast cancers. Therefore, we hypothesized that deregulated expression of SNX3 may change the dynamics of endocytic trafficking of membrane receptors in cancer cells. In order to test our hypothesis, we developed different RNA interference (RNAi) models of SNX3 in cancer cell lines and observed that WLS (Wntless, one of the known cargos of SNX3) and EGFR (Epidermal Growth Factor Receptor, possible cargo of SNX3) levels were altered in SNX3 silenced cells compared with the controls. We used proximity-based ligation approach to demonstrate possible interaction between SNX3 and EGFR, used immunostaining to determine subcellular localization of SNX3 and several mutants we generated that showed deregulated ubiquitylation and abnormal subcellular localization. Overall, we showed that SNX3 modulates EGFR protein levels and that ubiquitylation may be important for SNX3 function. Understanding the role of SNX3 and the endocytic trafficking may have future implications for targeted therapies against EGFR.
Subject Keywords
Growth factors
,
Growth factors Receptors.
,
Sorting Nexin 3
,
Epidermal Growth Factor Receptor
,
Wntless
,
Cancer
,
Ubiquitination
URI
http://etd.lib.metu.edu.tr/upload/12625238/index.pdf
https://hdl.handle.net/11511/45332
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Sorting nexin 3 (SNX3) is an immediate and sustained target of EGF stimulation in EGFR positive breast cells
Çiçek, Esra; Erson Bensan, Ayşe Elif; Department of Biology (2021-9-10)
Sorting nexin 3 (SNX3) is a member of the sorting nexins family, which is involved in the endocytic trafficking network. SNX3 only has a PX domain, which helps endosomes bind to phosphatidyl inositol-3-phosphate and plays a role in the constitution of the retromer complex on early endosomes. Endocytosed receptors are internalized and recycled back to the plasma membrane, the Trans-Golgi Network, or are degraded in the lysosomes. The current understanding of SNX3 function in recycling Epidermal Growth Factor...
Evaluation of crossover techniques in genetic algorithm based optimum structural design
Hasançebi, Oğuzhan (1998-08-21)
Crossover is one of the three basic operators in ally genetic algorithm (GA). Several crossover techniques have been proposed and their relative merits are currently under investigation. This paper starts with a brief discussion of the working scheme of genetic algorithms (GAs) and crossover techniques commonly used in previous GA applications. Next, these techniques are tested on two truss size optimization problems, and are evaluated with respect to exploration and exploitation aspects of the search proce...
Investigation of Stationarity for Graph Time Series Data Sets
Güneyi, Eylem Tuğçe; Vural, Elif (2021-01-07)
Graphs permit the analysis of the relationships in complex data sets effectively. Stationarity is a feature that facilitates the analysis and processing of random time signals. Since graphs have an irregular structure, the definition of classical stationarity does not apply to graphs. In this study, we study how stationarity is defined for graph random processes and examine the validity of the stationarity assumption with experiments on synthetic and real data sets.
A minimum distance bound for quasi-nD-cyclic codes
Özbudak, Ferruh (Elsevier BV, 2016-09-01)
We provide a new concatenated structure for multidimensional quasi-cyclic (QnDC) codes over F-q and we give a trace representation for their codewords, which extends the known representations of QC and nD cyclic codes. Based on these results, we obtain a minimum distance bound for QnDC dyclic codes. Since QnDC codes are naturally related to nD convolutional codes, this bound also applies to a special class of 1-generator 2D convolutional codes.
A note on Riesz spaces with property-b
Alpay, S.; Altin, B.; Tonyali, C. (Institute of Mathematics, Czech Academy of Sciences, 2006-01-01)
We study an order boundedness property in Riesz spaces and investigate Riesz spaces and Banach lattices enjoying this property.
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. Öyken, “Characterization of sorting nexin 3 (SNX3) function,” Thesis (Ph.D.) -- Graduate School of Natural and Applied Sciences. Biology., Middle East Technical University, 2020.