Phosphate enrichment and fed-batch operation for prolonged beta-lactamase production by Bacillus licheniformis

Ileri, N.
Çalık, Pınar
Sengul, A.
Aims: Investigation of the phosphate effect and feeding strategy, i.e. linear and exponential feeding, to improve beta-lactamase production by Bacillus licheniformis considering the viability of the cells.


Determination and metabolic engineering of rate limiting reactions in aromatic amino acid pathway in Bacillus subtilis for L-phenylaianine production
Guzide, Calik; Yasemin, Demirci; Pinar, Calik; Ozdamar, Tuncer H. (2007-09-01)
Rate limiting reactions in the aromatic-group amino acid pathway (AAAP) in Bacillus subtilis for l-phenylalanine (Phe) production were determined as reported elsewhere (Özçelik-Şenver et al., 2004). The biochemical reactions in the AAAP start with the reaction using phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P) synthesised in the glycolysis pathway and the PPP, respectively, leading to the formation of Phe via chorismate by branching at prephenate. The branch-point metabolites E4P supplied in vi...
Exponential feeding strategy development for benzaldehyde lyase production by recombinant "Escherichia coli"
Taşpınar, Hatice; Çalık, Pınar; Özdamar, Tunçer H.; Department of Biotechnology (2010)
In this study, the aim was to investigate the effects of exponential feeding strategy on benzaldehyde lyase (BAL) production by recombinant Escherichia coli BL21. For this purpose, the effects of medium components were investigated to optimize the initial medium composition of the fed-batch fermentations. For the batch bioreactor operations, the highest cell concentration and BAL activity were achieved in a media containing 30 g L-1 pretreated molasses, and 5 g L-1 (NH4)2HPO4 as 5.07 g L-1, and 1611 U ml-1 ...
Dynamic flux balance analysis for pharmaceutical protein production by Pichia pastoris: Human growth hormone
Çalık, Pınar; Taspinar, Hatice; Soyaslan, Elif S.; Inankur, Bahar (Elsevier BV, 2011-03-07)
The influence of methanol feeding rate on intracellular reaction network of recombinant human growth hormone (rhGH) producing Pichia pastoris was investigated at three different specific growth rates, namely, 0.02 (MS-0.02), 0.03 (MS-0.03), and 0.04h(-1) (MS-0.04) where Period-I (33 <= t < 42 h) includes the early exponential growth phase; Period-II (42 <= t < 48 h) is the exponential growth phase where the specific cell growth rate decreases; Period-III (48 <= t <= 51 h) is the exponential growth phase whe...
OZILGEN, M (Springer Science and Business Media LLC, 1988-12-01)
Large amounts of data indicated that most of the metabolic processes of the acidogenic (acid producing) and the solventogenic (solvent producing) fermentations were regulated by product accumulation. A simple unstructured model simulated microbial growth, product formation and substrate utilization in six different fermentations, where five different microorganisms produced various combinations of ten different products. Specific growth rates of these microorganisms decreased proportionally with overall pro...
Analysis of acyl CoA ester intermediates of the mevalonate pathway in Saccharomyces cerevisiae.
Şeker, Tamay; Nielsen, J (Springer Science and Business Media LLC, 2005-04-01)
The mevalonate pathway plays an important role in providing the cell with a number of essential precursors for the synthesis of biomass constituents. With respect to their chemical structure, the metabolites of this pathway can be divided into two groups: acyl esters [acetoacetyl CoA, acetyl CoA, hydroxymethylglutaryl (HMG) CoA] and phosphorylated metabolites (isopentenyl pyrophosphate, dimethylallyl pyrophosphate, geranyl pyrophosphate, farnesyl pyrophosphate). In this study, we developed a method for the ...
Citation Formats
N. Ileri, P. Çalık, and A. Sengul, “Phosphate enrichment and fed-batch operation for prolonged beta-lactamase production by Bacillus licheniformis,” JOURNAL OF APPLIED MICROBIOLOGY, pp. 1418–1426, 2007, Accessed: 00, 2020. [Online]. Available: