Synthesis and characterization of conducting polymers containing polypeptide and ferrocene side chains as ethanol biosensors

2014-01-01
KESIK, Melis
Akbulut, Huseyin
SOYLEMEZ, Saniye
CEVHER, ŞEVKİ CAN
HIZALAN, Gonul
Udum, Yasemin Arslan
ENDO, Takeshi
YAMADA, Shuhei
Çırpan, Ali
Yağcı, Yusuf
Toppare, Levent Kamil
A novel approach for the fabrication of a biosensor from a conducting polymer bearing polypeptide segments and ferrocene moieties is reported. The approach involves the electrochemical copolymerization of the electroactive polypeptide macromonomer and independently prepared ferrocene imidazole derivative of dithiophene, on the electrode surface. The polypeptide macromonomer was synthesized by the simultaneous formation of N-carboxyanhydride (NCA) and ring opening polymerization of N-Boc-L-lysine (alpha-amino acid of the corresponding NCA) using an amino functional bis-EDOT derivative (BEDOA-6) as an initiator. Alcohol oxidase was then covalently immobilized onto the copolymer coated electrode using glutaraldehyde as the crosslinking agent. The intermediates and final conducting copolymer before and after enzyme immobilization were fully characterized by FT-IR, H-1-NMR, GPC, cyclic voltammetry, SEM and EIS analyses. The newly designed biosensor which combined the advantages of each component was tested as an ethanol sensing system offering fast response time (9 s), wide linear range (0.17 mM and 4.25 mM) and low detection limit (0.28 mM) with a high sensitivity (12.52 mu A mM(-1) cm(-2)). Kinetic parameters K-M(app) and I-max were 2.67 mM and 2.98 mu A, respectively. The capability of the biosensor in determining ethanol content in alcoholic beverages was also demonstrated.
POLYMER CHEMISTRY

Suggestions

Synthesis and Characterization of a Luminol Based Chemiluminescent Trimeric System
Kesimal, Busra; Balci, Burcu; Çakal, Deniz; Önal, Ahmet Muhtar; Cihaner, Atilla (2023-01-01)
A luminol based chemiluminescent trimeric system, namely 2,3-dihydro-5,8-di(thiophen-2-yl)phthalazine-1,4-dione (T2B-Lum), bearing thiophene rings as donor units and 2,3-dihydrophthalazine-1,4-dione as an acceptor unit was synthesized in two steps via donor-acceptor-donor approach using two different methods. It was found that T2B-Lum emits chemiluminescent light when exposed to H2O2 in a basic medium, and the presence of substituents and the type of aromatic ring bearing chemiluminescent active group have ...
Conducting polymer composites of polypyrrole and polyimide
Selampinar, F; Akbulut, Ural; Toppare, Levent Kamil (1997-01-01)
A conducting composite of polypyrrole with a polyimide as the insulating matrix polymer was prepared via electrochemical methods. The characterization of the composite was done by FTIR, SEM and TGA studies. Conductivity and solubility studies together with spectroscopic methods reveal that a chemical interaction between the two polymers exists.
Synthesis, spectroscopic and electrochemical studies of perylene containing conjugated copolymers
Bayseç, Şebnem; Toppare, Levent Kamil; Yılmaz, Levent; Department of Polymer Science and Technology (2013)
The synthesis , spectroscopic and electrochemical properties of a series of donor-acceptor (DA) type polymers by changing the donor unit (thiophene, dithienopyrrole) polymer backbone were investigated.The synthesized CoP1 and CoP2 were designed to investigate the effect of copolymerization and insertion of perylene tetracarboxylic dimide (PDI) on the structure of benzotriazole (BTz) based polymers. For CoP1 1,7-dibromo-perylene diimide,4,7-dibromo-2-dodecyl-2H-benzo[d][1,2,3]triazole and 2,5 bis(tributylsta...
Design of Carbon Nanotube Modified Conducting Polymer for Biosensing Applications
Ozdemir, Caglar; Tuncagil, Sevinc; ODACI DEMİRKOL, DİLEK; TİMUR, SUNA; Toppare, Levent Kamil (2011-01-01)
An electrochemical biosensor with improved performance was designed through the immobilization of glucose oxidase (GOx) onto conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine (SNS-NH2) modified with carbon nanotubes (CNTs). For the optimization and characterization of the biosensor, pH profile, enzyme loading, reproducibility, operational stability experiments were carried out. It was found that the use of CNTs in a biosensing system enhanced the biosensor response. The linear relati...
Characterization of pea flour based nanofibers produced by electrospinning method
Oğuz, Seren; Şahin, Serpil; Şümnü, Servet Gülüm; Department of Food Engineering (2018)
Electrospinning is a process that produces continuous nanofibers through the action of an electric field imposed on a polymer solution. In this thesis, it was aimed to produce pea flour and hydroxypropyl methylcellulose (HPMC) based homogenous nanofibers by electrospinning. The effects of pH, pea flour and HPMC concentration, and microfluidization on apparent viscosity, electrical conductivity of solutions and nanofiber characteristics were studied. In addition, the effects of voltage and flow rate were ana...
Citation Formats
M. KESIK et al., “Synthesis and characterization of conducting polymers containing polypeptide and ferrocene side chains as ethanol biosensors,” POLYMER CHEMISTRY, pp. 6295–6306, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46053.