Synthesis and characterization of conducting polymers containing polypeptide and ferrocene side chains as ethanol biosensors

Date

2014-01-01

Author

KESIK, Melis
Akbulut, Huseyin
SOYLEMEZ, Saniye
CEVHER, ŞEVKİ CAN
HIZALAN, Gonul
Udum, Yasemin Arslan
ENDO, Takeshi
YAMADA, Shuhei
Çırpan, Ali
Yağcı, Yusuf
Toppare, Levent Kamil

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A novel approach for the fabrication of a biosensor from a conducting polymer bearing polypeptide segments and ferrocene moieties is reported. The approach involves the electrochemical copolymerization of the electroactive polypeptide macromonomer and independently prepared ferrocene imidazole derivative of dithiophene, on the electrode surface. The polypeptide macromonomer was synthesized by the simultaneous formation of N-carboxyanhydride (NCA) and ring opening polymerization of N-Boc-L-lysine (alpha-amino acid of the corresponding NCA) using an amino functional bis-EDOT derivative (BEDOA-6) as an initiator. Alcohol oxidase was then covalently immobilized onto the copolymer coated electrode using glutaraldehyde as the crosslinking agent. The intermediates and final conducting copolymer before and after enzyme immobilization were fully characterized by FT-IR, H-1-NMR, GPC, cyclic voltammetry, SEM and EIS analyses. The newly designed biosensor which combined the advantages of each component was tested as an ethanol sensing system offering fast response time (9 s), wide linear range (0.17 mM and 4.25 mM) and low detection limit (0.28 mM) with a high sensitivity (12.52 mu A mM(-1) cm(-2)). Kinetic parameters K-M(app) and I-max were 2.67 mM and 2.98 mu A, respectively. The capability of the biosensor in determining ethanol content in alcoholic beverages was also demonstrated.

Subject Keywords

Electrochemical impedance spectroscopy, Activated urethane derivatives, N-carboxyanhydride, Alcohol oxidase, Glucose-oxidase, L-glutamate, Thiophene, Nanoparticles, Immobilization

URI

https://hdl.handle.net/11511/46053

Journal

POLYMER CHEMISTRY

DOI

https://doi.org/10.1039/c4py00850b

Collections

Department of Chemistry, Article

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Citation Formats

M. KESIK et al., “Synthesis and characterization of conducting polymers containing polypeptide and ferrocene side chains as ethanol biosensors,” POLYMER CHEMISTRY, pp. 6295–6306, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46053.