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REGULATION OF BIOSYNTHESIS OF BACILYSIN BY BACILLUS-SUBTILIS
Date
1990-10-01
Author
Özcengiz, Gülay
DEMAIN, AL
Metadata
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Subject Keywords
Biotechnology
,
Applied Microbiology and Biotechnology
URI
https://hdl.handle.net/11511/46650
Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY
DOI
https://doi.org/10.1007/bf01576428
Collections
Department of Biology, Article
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Role of the cmcH-ccaR intergenic region and ccaR overexpression in cephamycin C biosynthesis in Streptomyces clavuligerus
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The effect of the CcaR regulatory protein on expression of the cephamycin C gene cluster is studied. Quantitative reverse transcription PCR (qRT-PCR) expression analysis of the cephamycin biosynthesis genes in the ccaR-disrupted strain, S. clavuligerus ccaR::aph, revealed that in the absence of CcaR, the lat and cmcI genes expression was reduced 2,200-and 1,087-fold compared with the wild type. Expression of pcbAB-pcbC-cefD-cefE-cmcJ-cmcH and blp was 225- to 359-fold lower, while expression of pcbR-pbpA-bla...
Targeted disruption of homoserine dehydrogenase gene and its effect on cephamycin C production in Streptomyces clavuligerus
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The aspartate pathway of Streptomyces clavuligerus is an important primary metabolic pathway which provides substrates for beta-lactam synthesis. In this study, the hom gene which encodes homoserine dehydrogenase was cloned from the cephamycin C producer S. clavuligerus NRRL 3585 and characterized. The fully sequenced open reading frame encodes 433 amino acids with a deduced M (r) of 44.9 kDa. The gene was heterologously expressed in the auxotroph mutant Escherichia coli CGSC 5075 and the recombinant protei...
Expression of chitinase A (chiA) gene from a local isolate of Serratia marcescens in Coleoptera-specific Bacillus thuringiensis
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Effects of exponential feeding strategy on benzaldehyde lyase production by recombinant Escherichia coli
Taspinar, Hatice; Çalık, Pınar (2010-11-01)
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G. Özcengiz and A. DEMAIN, “REGULATION OF BIOSYNTHESIS OF BACILYSIN BY BACILLUS-SUBTILIS,”
JOURNAL OF INDUSTRIAL MICROBIOLOGY
, pp. 91–100, 1990, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46650.